Syk Plays a Critical Role in the Expression and Activation of IRAK1 in LPS-Treated Macrophages
المؤلفون المشاركون
Yang, Woo Seok
Kim, Ji Hye
Yoo, Byong Chul
Kim, Jong-Hoon
Cho, Jae Youl
Park, Jae Gwang
Son, Young-Jin
المصدر
العدد
المجلد 2017، العدد 2017 (31 ديسمبر/كانون الأول 2017)، ص ص. 1-9، 9ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2017-06-07
دولة النشر
مصر
عدد الصفحات
9
التخصصات الرئيسية
الملخص EN
To address how interleukin-1 receptor-associated kinase 1 (IRAK1) is controlled by other enzymes activated by toll-like receptor (TLR) 4, we investigated the possibility that spleen tyrosine kinase (Syk), a protein tyrosine kinase that is activated at an earlier stage during TLR4 activation, plays a central role in regulating the functional activation of IRAK1.
Indeed, we found that overexpression of myeloid differentiation primary response gene 88 (MyD88), an adaptor molecule that drives TLR signaling, induced IRAK1 expression and that piceatannol, a Syk inhibitor, successfully suppressed the MyD88-dependent upregulation of IRAK1 under LPS treatment conditions.
Interestingly, in Syk-knockout RAW264.7 cells, IRAK1 activity was almost completely blocked after LPS treatment, while providing a Syk-recovery gene to the knockout cells successfully restored IRAK1 expression.
According to our measurements of IRAK1 mRNA levels, the transcriptional upregulation of IRAK1 was induced by LPS treatment between 4 and 60 min, and this can be suppressed in Syk knockout cells, providing an effect similar that that seen under piceatannol treatment.
The overexpression of Syk reverses this effect and leads to a significantly higher IRAK1 mRNA level.
Collectively, our results strongly suggest that Syk plays a critical role in regulating both the activity and transcriptional level of IRAK1.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Park, Jae Gwang& Son, Young-Jin& Yoo, Byong Chul& Yang, Woo Seok& Kim, Ji Hye& Kim, Jong-Hoon…[et al.]. 2017. Syk Plays a Critical Role in the Expression and Activation of IRAK1 in LPS-Treated Macrophages. Mediators of Inflammation،Vol. 2017, no. 2017, pp.1-9.
https://search.emarefa.net/detail/BIM-1188084
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Park, Jae Gwang…[et al.]. Syk Plays a Critical Role in the Expression and Activation of IRAK1 in LPS-Treated Macrophages. Mediators of Inflammation No. 2017 (2017), pp.1-9.
https://search.emarefa.net/detail/BIM-1188084
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Park, Jae Gwang& Son, Young-Jin& Yoo, Byong Chul& Yang, Woo Seok& Kim, Ji Hye& Kim, Jong-Hoon…[et al.]. Syk Plays a Critical Role in the Expression and Activation of IRAK1 in LPS-Treated Macrophages. Mediators of Inflammation. 2017. Vol. 2017, no. 2017, pp.1-9.
https://search.emarefa.net/detail/BIM-1188084
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1188084
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر