Proinflammatory Cytokines IL-6 and TNF-α Increased Telomerase Activity through NF-κBSTAT1STAT3 Activation, and Withaferin A Inhibited the Signaling in Colorectal Cancer Cells
المؤلفون المشاركون
Vadgama, Jaydutt
Wu, Yong
Atefi, Mohammad
Chung, Seyung S.
Okobi, Quincy
Adekoya, Debbie
Clarke, Orette
Dutta, Pranabananda
المصدر
العدد
المجلد 2017، العدد 2017 (31 ديسمبر/كانون الأول 2017)، ص ص. 1-11، 11ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2017-06-06
دولة النشر
مصر
عدد الصفحات
11
التخصصات الرئيسية
الملخص EN
There are increasing evidences of proinflammatory cytokine involvement in cancer development.
Here, we found that two cytokines, IL-6 and TNF-α, activated colorectal cancer cells to be more invasive and stem-like.
Combined treatment of IL-6 and TNF-α phosphorylated transcription factors STAT3 in a synergistic manner.
STAT3, STAT1, and NF-κB physically interacted upon the cytokine stimulation.
STAT3 was bound to the promoter region of human telomerase reverse transcriptase (hTERT).
IL-6 and TNF-α stimulation further enhanced STAT3 binding affinity.
Stem cell marker Oct-4 was upregulated in colorectal cancer cells upon IL-6 and TNF-α stimulation.
Withaferin A, an anti-inflammatory steroidal lactone, inhibited the IL-6- and TNF-α-induced cancer cell invasion and decreased colonosphere formation.
Notably, withaferin A inhibited STAT3 phosphorylation and abolished the STAT3, STAT1, and NF-κB interactions.
Oct-4 expression was also downregulated by withaferin A inhibition.
The binding of STAT3 to the hTERT promoter region and telomerase activity showed reduction with withaferin A treatments.
Proinflammatory cytokine-induced cancer cell invasiveness is mediated by a STAT3-regulated mechanism in colorectal cancer cells.
Our data suggest that withaferin A could be a promising anticancer agent that effectively inhibits the progression of colorectal cancer.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Chung, Seyung S.& Wu, Yong& Okobi, Quincy& Adekoya, Debbie& Atefi, Mohammad& Clarke, Orette…[et al.]. 2017. Proinflammatory Cytokines IL-6 and TNF-α Increased Telomerase Activity through NF-κBSTAT1STAT3 Activation, and Withaferin A Inhibited the Signaling in Colorectal Cancer Cells. Mediators of Inflammation،Vol. 2017, no. 2017, pp.1-11.
https://search.emarefa.net/detail/BIM-1188518
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Chung, Seyung S.…[et al.]. Proinflammatory Cytokines IL-6 and TNF-α Increased Telomerase Activity through NF-κBSTAT1STAT3 Activation, and Withaferin A Inhibited the Signaling in Colorectal Cancer Cells. Mediators of Inflammation No. 2017 (2017), pp.1-11.
https://search.emarefa.net/detail/BIM-1188518
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Chung, Seyung S.& Wu, Yong& Okobi, Quincy& Adekoya, Debbie& Atefi, Mohammad& Clarke, Orette…[et al.]. Proinflammatory Cytokines IL-6 and TNF-α Increased Telomerase Activity through NF-κBSTAT1STAT3 Activation, and Withaferin A Inhibited the Signaling in Colorectal Cancer Cells. Mediators of Inflammation. 2017. Vol. 2017, no. 2017, pp.1-11.
https://search.emarefa.net/detail/BIM-1188518
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1188518
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر