Beneficial Effects of Human Mesenchymal Stromal Cells on Porcine Hepatocyte Viability and Albumin Secretion
المؤلفون المشاركون
Montanari, Elisa
Pimenta, Joel
Szabó, Luca
Noverraz, François
Passemard, Solène
Meier, Raphael P. H.
Meyer, Jeremy
Sidibe, Jonathan
Thomas, Aurelien
Schuurman, Henk-Jan
Gerber-Lemaire, Sandrine
Gonelle-Gispert, Carmen
Buhler, Leo H.
المصدر
Journal of Immunology Research
العدد
المجلد 2018، العدد 2018 (31 ديسمبر/كانون الأول 2018)، ص ص. 1-13، 13ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2018-01-11
دولة النشر
مصر
عدد الصفحات
13
التخصصات الرئيسية
الملخص EN
Porcine hepatocytes transplanted during acute liver failure might support metabolic functions until the diseased liver recovers its function.
Here, we isolated high numbers of viable pig hepatocytes and evaluated hepatocyte functionality after encapsulation.
We further investigated whether coculture and coencapsulation of hepatocytes with human multipotent mesenchymal stromal cells (MSC) are beneficial on hepatocyte function.
Livers from 10 kg pigs (n=9) were harvested, and hepatocytes were isolated from liver suspensions for microencapsulation using alginate and poly(ethylene-glycol)- (PEG-) grafted alginate hydrogels, either alone or in combination with MSC.
Viability, albumin secretion, and diazepam catabolism of hepatocytes were measured for one week.
9.2 ± 3.6 × 109 hepatocytes with 95.2 ± 3.1% viability were obtained after isolation.
At day 3, free hepatocytes displayed 99% viability, whereas microencapsulation in alginate and PEG-grafted alginate decreased viability to 62% and 48%, respectively.
Albumin secretion and diazepam catabolism occurred in free and microencapsulated hepatocytes.
Coencapsulation of hepatocytes with MSC significantly improved viability and albumin secretion at days 4 and 8 (p<0.05).
Coculture with MSC significantly increased and prolonged albumin secretion.
In conclusion, we established a protocol for isolation and microencapsulation of high numbers of viable pig hepatocytes and demonstrated that the presence of MSC is beneficial for the viability and function of porcine hepatocytes.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Montanari, Elisa& Pimenta, Joel& Szabó, Luca& Noverraz, François& Passemard, Solène& Meier, Raphael P. H.…[et al.]. 2018. Beneficial Effects of Human Mesenchymal Stromal Cells on Porcine Hepatocyte Viability and Albumin Secretion. Journal of Immunology Research،Vol. 2018, no. 2018, pp.1-13.
https://search.emarefa.net/detail/BIM-1191528
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Montanari, Elisa…[et al.]. Beneficial Effects of Human Mesenchymal Stromal Cells on Porcine Hepatocyte Viability and Albumin Secretion. Journal of Immunology Research No. 2018 (2018), pp.1-13.
https://search.emarefa.net/detail/BIM-1191528
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Montanari, Elisa& Pimenta, Joel& Szabó, Luca& Noverraz, François& Passemard, Solène& Meier, Raphael P. H.…[et al.]. Beneficial Effects of Human Mesenchymal Stromal Cells on Porcine Hepatocyte Viability and Albumin Secretion. Journal of Immunology Research. 2018. Vol. 2018, no. 2018, pp.1-13.
https://search.emarefa.net/detail/BIM-1191528
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1191528
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر