Atorvastatin and Conditioned Media from Atorvastatin-Treated Human Hematopoietic StemProgenitor-Derived Cells Show Proangiogenic Activity In Vitro but Not In Vivo
المؤلفون المشاركون
Florczyk, Urszula
Józkowicz, Alicja
Dulak, Jozef
Nowak, Witold N.
Nizankowski, Rafal
Taha, Hevidar
Markiewicz, Joanna
Kachamakova-Trojanowska, Neli
Stępniewski, Jacek
Klóska, Damian
Frołow, Marzena
Walter, Zbigniew
المصدر
العدد
المجلد 2019، العدد 2019 (31 ديسمبر/كانون الأول 2019)، ص ص. 1-15، 15ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2019-07-16
دولة النشر
مصر
عدد الصفحات
15
التخصصات الرئيسية
الملخص EN
Myeloid angiogenic cells (MAC) derive from hematopoietic stem/progenitor cells (HSPCs) that are mobilized from the bone marrow.
They home to sites of neovascularization and contribute to angiogenesis by production of paracrine factors.
The number and function of proangiogenic cells are impaired in patients with diabetes or cardiovascular diseases.
Both conditions can be accompanied by decreased levels of heme oxygenase-1 (HMOX1), cytoprotective, heme-degrading enzyme.
Our study is aimed at investigating whether precursors of myeloid angiogenic cells (PACs) treated with known pharmaceuticals would produce media with better proangiogenic activity in vitro and if such media can be used to stimulate blood vessel growth in vivo.
We used G-CSF-mobilized CD34+ HSPCs, FACS-sorted from healthy donor peripheral blood mononuclear cells (PBMCs).
Sorted cells were predominantly CD133+.
CD34+ cells after six days in culture were stimulated with atorvastatin (AT), acetylsalicylic acid (ASA), sulforaphane (SR), resveratrol (RV), or metformin (Met) for 48 h.
Conditioned media from such cells were then used to stimulate human aortic endothelial cells (HAoECs) to enhance tube-like structure formation in a Matrigel assay.
The only stimulant that enhanced PAC paracrine angiogenic activity was atorvastatin, which also had ability to stabilize endothelial tubes in vitro.
On the other hand, the only one that induced heme oxygenase-1 expression was sulforaphane, a known activator of a HMOX1 inducer—NRF2.
None of the stimulants changed significantly the levels of 30 cytokines and growth factors tested with the multiplex test.
Then, we used atorvastatin-stimulated cells or conditioned media from them in the Matrigel plug in vivo angiogenic assay.
Neither AT alone in control media nor conditioned media nor AT-stimulated cells affected numbers of endothelial cells in the plug or plug’s vascularization.
Concluding, high concentrations of atorvastatin stabilize tubes and enhance the paracrine angiogenic activity of human PAC cells in vitro.
However, the effect was not observed in vivo.
Therefore, the use of conditioned media from atorvastatin-treated PAC is not a promising therapeutic strategy to enhance angiogenesis.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Nowak, Witold N.& Taha, Hevidar& Markiewicz, Joanna& Kachamakova-Trojanowska, Neli& Stępniewski, Jacek& Klóska, Damian…[et al.]. 2019. Atorvastatin and Conditioned Media from Atorvastatin-Treated Human Hematopoietic StemProgenitor-Derived Cells Show Proangiogenic Activity In Vitro but Not In Vivo. Mediators of Inflammation،Vol. 2019, no. 2019, pp.1-15.
https://search.emarefa.net/detail/BIM-1192643
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Nowak, Witold N.…[et al.]. Atorvastatin and Conditioned Media from Atorvastatin-Treated Human Hematopoietic StemProgenitor-Derived Cells Show Proangiogenic Activity In Vitro but Not In Vivo. Mediators of Inflammation No. 2019 (2019), pp.1-15.
https://search.emarefa.net/detail/BIM-1192643
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Nowak, Witold N.& Taha, Hevidar& Markiewicz, Joanna& Kachamakova-Trojanowska, Neli& Stępniewski, Jacek& Klóska, Damian…[et al.]. Atorvastatin and Conditioned Media from Atorvastatin-Treated Human Hematopoietic StemProgenitor-Derived Cells Show Proangiogenic Activity In Vitro but Not In Vivo. Mediators of Inflammation. 2019. Vol. 2019, no. 2019, pp.1-15.
https://search.emarefa.net/detail/BIM-1192643
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1192643
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر