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PPAR γNnatNF-κB Axis Involved in Promoting Effects of Adiponectin on Preadipocyte Differentiation
المؤلفون المشاركون
Yang, Wenkai
Yuan, Wenjin
Peng, Xinghua
Wang, Meiling
Xiao, Jie
Wu, Cheng
Luo, Lie
المصدر
العدد
المجلد 2019، العدد 2019 (31 ديسمبر/كانون الأول 2019)، ص ص. 1-9، 9ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2019-11-21
دولة النشر
مصر
عدد الصفحات
9
التخصصات الرئيسية
الملخص EN
A previous study has demonstrated that adiponectin (APN) could promote preadipocyte differentiation, and the present study further explored its mechanism.
3T3-L1 cells were infected with adenovirus holding human adiponectin gene apM1 and mouse neuronatin (Nnat) shRNA and initiated differentiation while coculturing with mature adipocytes stimulated with LPS.
After 8 days, preadipocyte differentiation was observed by Oil Red O staining.
Real-time quantitative PCR was used to evaluate mRNA expression levels of monocyte chemoattractant protein-1 (MCP-1), interleukin- (IL-) 6, IL-8, and tumor necrosis factor α (TNF-α).
The levels of reactive oxygen species (ROS), total antioxidant capacity (T-AOC), malondialdehyde (MDA), and superoxide dismutase (SOD) in 3T3-L1 cells were detected.
Western blotting was done to quantify the protein expression levels of Nnat, peroxisome proliferator-activated receptor (PPAR) γ, p65, and inhibitor of nuclear factor κB (IκB) α.
Results demonstrated that APN overexpression markedly increased preadipocyte differentiation; inhibited gene expression of MCP-1, IL-6, IL-8, and TNF-α; reduced ROS and MDA release; increased T-AOC and SOD levels; upregulated Nnat, PPAR γ, and IκB α protein expressions; and downregulated p65 protein expression under LPS stimulation.
However, the effects of APN were markedly attenuated when Nnat expression was knocked down.
Taken together, the present study provided evidences that the effects of APN on promoting preadipocyte differentiation under inflammatory conditions via anti-inflammation and antioxidative stress may be regulated by the PPAR γ/Nnat/NF-κB signaling pathway.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Yang, Wenkai& Yuan, Wenjin& Peng, Xinghua& Wang, Meiling& Xiao, Jie& Wu, Cheng…[et al.]. 2019. PPAR γNnatNF-κB Axis Involved in Promoting Effects of Adiponectin on Preadipocyte Differentiation. Mediators of Inflammation،Vol. 2019, no. 2019, pp.1-9.
https://search.emarefa.net/detail/BIM-1192958
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Yang, Wenkai…[et al.]. PPAR γNnatNF-κB Axis Involved in Promoting Effects of Adiponectin on Preadipocyte Differentiation. Mediators of Inflammation No. 2019 (2019), pp.1-9.
https://search.emarefa.net/detail/BIM-1192958
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Yang, Wenkai& Yuan, Wenjin& Peng, Xinghua& Wang, Meiling& Xiao, Jie& Wu, Cheng…[et al.]. PPAR γNnatNF-κB Axis Involved in Promoting Effects of Adiponectin on Preadipocyte Differentiation. Mediators of Inflammation. 2019. Vol. 2019, no. 2019, pp.1-9.
https://search.emarefa.net/detail/BIM-1192958
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1192958
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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