Role of the Btk-PLCγ2 Signaling Pathway in the Bone Destruction of Apical Periodontitis
المؤلفون المشاركون
Wang, Lina
Zhang, Hong
Dong, Ming
Zuo, Meina
Liu, Shuo
Lu, Ying
Niu, Weidong
المصدر
العدد
المجلد 2019، العدد 2019 (31 ديسمبر/كانون الأول 2019)، ص ص. 1-10، 10ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2019-07-25
دولة النشر
مصر
عدد الصفحات
10
التخصصات الرئيسية
الملخص EN
Chronic apical periodontitis is characterized by alveolar bone absorption in the apical region and is the result of the participation of various inflammatory mediators.
Studies have shown that the Bruton tyrosine kinase- (Btk-) phospholipase Cγ2 (PLCγ2) signaling pathway plays an important role in bone absorption, but it is unknown whether it plays a role in apical periodontitis bone destruction.
Therefore, this study verified the role of Btk and PLCγ2 in bone resorption of apical periodontitis by in vivo and in vitro experiments.
In the in vivo experiment, a mice model of apical periodontitis was established; apical bone resorption was confirmed by the numbers of osteoclasts and HE staining.
Btk, PLCγ2, and nuclear factor of activated T-cells 1 (NFATc-1) were detected by immunohistochemical staining.
In the in vitro experiment, lipopolysaccharides (LPS) were used to stimulate osteoclast precursor cell RAW264.7 to establish an inflammatory microenvironment and detect osteoclast differentiation.
By silencing Btk, the expression of Btk, PLCγ2, and NFATc-1 was detected by real-time qPCR and Western blot, and osteoclastogenesis was detected by enzyme histochemical staining to further confirm the role of Btk in bone resorption.
It was found that the expression of Btk, PLCγ2, and NFATc-1 changed significantly with the progression of inflammation and bone destruction, indicating that Btk and PLCγ2 may be involved in the progression of inflammation in apical periodontitis and bone absorption.
In vitro experiments confirmed that the differentiation of osteoclasts and the expression of PLCγ2 and NFATc-1 were significantly inhibited after silencing Btk expression, but osteoclast precursor cells could be differentiated due to the proinflammatory factor lipopolysaccharide.
This study demonstrates that Btk and PLCγ2 are key factors involved in the apical inflammatory response and bone destruction.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Wang, Lina& Zhang, Hong& Dong, Ming& Zuo, Meina& Liu, Shuo& Lu, Ying…[et al.]. 2019. Role of the Btk-PLCγ2 Signaling Pathway in the Bone Destruction of Apical Periodontitis. Mediators of Inflammation،Vol. 2019, no. 2019, pp.1-10.
https://search.emarefa.net/detail/BIM-1193582
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Wang, Lina…[et al.]. Role of the Btk-PLCγ2 Signaling Pathway in the Bone Destruction of Apical Periodontitis. Mediators of Inflammation No. 2019 (2019), pp.1-10.
https://search.emarefa.net/detail/BIM-1193582
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Wang, Lina& Zhang, Hong& Dong, Ming& Zuo, Meina& Liu, Shuo& Lu, Ying…[et al.]. Role of the Btk-PLCγ2 Signaling Pathway in the Bone Destruction of Apical Periodontitis. Mediators of Inflammation. 2019. Vol. 2019, no. 2019, pp.1-10.
https://search.emarefa.net/detail/BIM-1193582
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1193582
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر