Evaluation of TGF-Beta 2 and VEGFα Gene Expression Levels in Epiretinal Membranes and Internal Limiting Membranes in the Course of Retinal Detachments, Proliferative Diabetic Retinopathy, Macular Holes, and Idiopathic Epiretinal Membranes
المؤلفون المشاركون
Stafiej, Joanna
Malukiewicz, Grażyna
Linkowska, Katarzyna
Grzybowski, Tomasz
Żuchowski, Paweł
Kaźmierczak, Karolina
المصدر
العدد
المجلد 2018، العدد 2018 (31 ديسمبر/كانون الأول 2018)، ص ص. 1-6، 6ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2018-04-23
دولة النشر
مصر
عدد الصفحات
6
التخصصات الرئيسية
الملخص EN
Purpose.
To evaluate the expression profiles of the VEGFα and TGFβ in the ERMs and ILMs in retinal disorders.
Methods.
In this nonrandomized prospective study, 75 patients (34 females and 41 males) referred to pars plana vitrectomy (PPV) due to different retinal diseases were enrolled to the study.
The samples of ERMs and ILMs collected during PPV were immediately put in TRIzol® Reagent (Life Technologies, USA) and stored at −70°C until RNA extraction.
Gene expression analysis was done with TaqMan® Gene Expression Assays (Applied Biosystems, USA) following the manufacturer’s instructions.
Results.
The gene expression levels of VEGFα as well as of TGFβ2 were significantly higher in ERMs than in ILMs in all studied groups.
The level of TGFβ2 expression exhibits a significantly lower values in iERMs as compared with the RRD group (p=0.043).
There were differences in TGFβ2 expression in ILM in groups studied: DR versus RRD, p=0.003; DR versus iERM, p=0,047; and iERM versus RRD, p=0.004.
Conclusions.
Our results revealed that factors associated with angiogenesis and wound healing processes in eyes with RRD, PDR, iERM, and MH were more upregulated in ERMs than in ILMs.
This may indicate that ILM is not responsible for reproliferation and its peeling should be avoided in routine PPV.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Stafiej, Joanna& Kaźmierczak, Karolina& Linkowska, Katarzyna& Żuchowski, Paweł& Grzybowski, Tomasz& Malukiewicz, Grażyna. 2018. Evaluation of TGF-Beta 2 and VEGFα Gene Expression Levels in Epiretinal Membranes and Internal Limiting Membranes in the Course of Retinal Detachments, Proliferative Diabetic Retinopathy, Macular Holes, and Idiopathic Epiretinal Membranes. Journal of Ophthalmology،Vol. 2018, no. 2018, pp.1-6.
https://search.emarefa.net/detail/BIM-1196971
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Stafiej, Joanna…[et al.]. Evaluation of TGF-Beta 2 and VEGFα Gene Expression Levels in Epiretinal Membranes and Internal Limiting Membranes in the Course of Retinal Detachments, Proliferative Diabetic Retinopathy, Macular Holes, and Idiopathic Epiretinal Membranes. Journal of Ophthalmology No. 2018 (2018), pp.1-6.
https://search.emarefa.net/detail/BIM-1196971
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Stafiej, Joanna& Kaźmierczak, Karolina& Linkowska, Katarzyna& Żuchowski, Paweł& Grzybowski, Tomasz& Malukiewicz, Grażyna. Evaluation of TGF-Beta 2 and VEGFα Gene Expression Levels in Epiretinal Membranes and Internal Limiting Membranes in the Course of Retinal Detachments, Proliferative Diabetic Retinopathy, Macular Holes, and Idiopathic Epiretinal Membranes. Journal of Ophthalmology. 2018. Vol. 2018, no. 2018, pp.1-6.
https://search.emarefa.net/detail/BIM-1196971
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1196971
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر