Quercetin and Quercetin-Rich Red Onion Extract Alter Pgc-1α Promoter Methylation and Splice Variant Expression

المؤلفون المشاركون

Devarshi, Prasad P.
Jones, Aarin D.
Taylor, Erin M.
Stefanska, Barbara
Henagan, Tara M.

المصدر

PPAR Research

العدد

المجلد 2017، العدد 2017 (31 ديسمبر/كانون الأول 2017)، ص ص. 1-8، 8ص.

الناشر

Hindawi Publishing Corporation

تاريخ النشر

2017-01-16

دولة النشر

مصر

عدد الصفحات

8

التخصصات الرئيسية

الأحياء

الملخص EN

Pgc-1α and its various isoforms may play a role in determining skeletal muscle mitochondrial adaptations in response to diet.

8 wks of dietary supplementation with the flavonoid quercetin (Q) or red onion extract (ROE) in a high fat diet (HFD) ameliorates HFD-induced obesity and insulin resistance in C57BL/J mice while upregulating Pgc-1α and increasing skeletal muscle mitochondrial number and function.

Here, mice were fed a low fat (LF), high fat (HF), high fat plus quercetin (HF + Q), or high fat plus red onion extract (HF + RO) diet for 9 wks and skeletal muscle Pgc-1α isoform expression and DNA methylation were determined.

Quantification of various Pgc-1α isoforms, including isoforms Pgc-1α-a, Pgc-1α-b, Pgc-1α-c, Pgc-1α4, total NT-Pgc-1α, and FL-Pgc-1α, showed that only total NT-Pgc-1α expression was increased in LF, HF + Q, and HF + RO compared to HF.

Furthermore, Q supplementation decreased Pgc-1α-a expression compared to LF and HF, and ROE decreased Pgc-1α-a expression compared to LF.

FL-Pgc-1α was decreased in HF + Q and HF + RO compared to LF and HF.

HF exhibited hypermethylation at the −260 nucleotide (nt) in the Pgc-1α promoter.

Q and ROE prevented HFD-induced hypermethylation.

−260 nt methylation levels were associated with NT-Pgc-1α expression only.

Pgc-1α isoform expression may be epigenetically regulated by Q and ROE through DNA methylation.

نمط استشهاد جمعية علماء النفس الأمريكية (APA)

Devarshi, Prasad P.& Jones, Aarin D.& Taylor, Erin M.& Stefanska, Barbara& Henagan, Tara M.. 2017. Quercetin and Quercetin-Rich Red Onion Extract Alter Pgc-1α Promoter Methylation and Splice Variant Expression. PPAR Research،Vol. 2017, no. 2017, pp.1-8.
https://search.emarefa.net/detail/BIM-1197216

نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)

Devarshi, Prasad P.…[et al.]. Quercetin and Quercetin-Rich Red Onion Extract Alter Pgc-1α Promoter Methylation and Splice Variant Expression. PPAR Research No. 2017 (2017), pp.1-8.
https://search.emarefa.net/detail/BIM-1197216

نمط استشهاد الجمعية الطبية الأمريكية (AMA)

Devarshi, Prasad P.& Jones, Aarin D.& Taylor, Erin M.& Stefanska, Barbara& Henagan, Tara M.. Quercetin and Quercetin-Rich Red Onion Extract Alter Pgc-1α Promoter Methylation and Splice Variant Expression. PPAR Research. 2017. Vol. 2017, no. 2017, pp.1-8.
https://search.emarefa.net/detail/BIM-1197216

نوع البيانات

مقالات

لغة النص

الإنجليزية

الملاحظات

Includes bibliographical references

رقم السجل

BIM-1197216