Propagation and Molecular Characterization of Bioreactor Adapted Very Virulent Infectious Bursal Disease Virus Isolates of Malaysia
المؤلفون المشاركون
Ideris, Aini
Arshad, Siti Suri
Hair-Bejo, Mohd
Lawal, Nafi’u
Omar, Abdul Rahman
المصدر
العدد
المجلد 2018، العدد 2018 (31 ديسمبر/كانون الأول 2018)، ص ص. 1-11، 11ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2018-09-02
دولة النشر
مصر
عدد الصفحات
11
التخصصات الرئيسية
الملخص EN
Two Malaysian very virulent infectious bursal disease virus (vvIBDV) strains UPM0081 (also known as B00/81) and UPM190 (also known as UPM04/190) isolated from local IBD outbreaks in 2000 and 2004, respectively, were separately passaged for 12 consecutive times in 11-day-old specific pathogen free (SPF) chicken embryonated eggs (CEE) via the chorioallantoic membrane (CAM) route.
The CEE passage 8 (EP8) isolates were passaged once in BGM-70 cell line yielding UPM0081EP8BGMP1 and UPM190EP8BGMP1, while the EP12 isolates were passaged 15 times in BGM-70 cell line yielding UPM0081EP12BGMP15 and UPM190EP12BGMP15 using T25 tissue culture flask.
These isolates were all propagated once in bioreactor using cytodex 1 as microcarrier at 3 g per liter (3 g/L) yielding UPM0081EP8BGMP1BP1, UPM190EP8BGMP1BP1, UPM0081EP12BGMP15BP1, and UPM190EP12BGMP15BP1 isolates.
The viruses were harvested at 3 days after inoculation, following the appearance of cytopathic effects (CPE) characterized by detachment from the microcarrier using standard protocol and filtered using 0.2 μm syringe filter.
The filtrates were positive for IBDV by RT-PCR and immunofluorescence.
Sequence and phylogenetic tree analysis indicated that the isolates were of the vvIBDV strains and were not different from the flask propagated parental viruses.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Lawal, Nafi’u& Hair-Bejo, Mohd& Arshad, Siti Suri& Omar, Abdul Rahman& Ideris, Aini. 2018. Propagation and Molecular Characterization of Bioreactor Adapted Very Virulent Infectious Bursal Disease Virus Isolates of Malaysia. Journal of Pathogens،Vol. 2018, no. 2018, pp.1-11.
https://search.emarefa.net/detail/BIM-1197443
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Lawal, Nafi’u…[et al.]. Propagation and Molecular Characterization of Bioreactor Adapted Very Virulent Infectious Bursal Disease Virus Isolates of Malaysia. Journal of Pathogens No. 2018 (2018), pp.1-11.
https://search.emarefa.net/detail/BIM-1197443
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Lawal, Nafi’u& Hair-Bejo, Mohd& Arshad, Siti Suri& Omar, Abdul Rahman& Ideris, Aini. Propagation and Molecular Characterization of Bioreactor Adapted Very Virulent Infectious Bursal Disease Virus Isolates of Malaysia. Journal of Pathogens. 2018. Vol. 2018, no. 2018, pp.1-11.
https://search.emarefa.net/detail/BIM-1197443
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1197443
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر