Maintenance of a Schwann-Like Phenotype in Differentiated Adipose-Derived Stem Cells Requires the Synergistic Action of Multiple Growth Factors
المؤلفون المشاركون
Mortimer, Alice E.
Kilic, Mahmut A.
Reid, Adam J.
Faroni, Alessandro
المصدر
العدد
المجلد 2017، العدد 2017 (31 ديسمبر/كانون الأول 2017)، ص ص. 1-7، 7ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2017-07-16
دولة النشر
مصر
عدد الصفحات
7
الملخص EN
Differentiating human adipose-derived stem cells (ASCs) towards Schwann cells produces an unstable phenotype when stimulating factors are withdrawn.
Here, we set out to examine the role of glial growth factor 2 (GGF-2) in the maintenance of Schwann-like cells.
Following ASC differentiation to Schwann-like cells, stimulating factors were withdrawn such that cells either remained in media supplemented with all stimulating factors, GGF-2 alone, or underwent complete withdrawal of all factors.
Furthermore, each stimulating factor was also removed from the growth medium individually.
At 72 hours, gene (qRT-PCR) and protein (ELISA) expression of key Schwann cell factors were quantified and cell morphology was analysed.
Cells treated with GGF-2 alone reverted to a stem cell morphology and did not stimulate the production of brain-derived neurotrophic factor (BDNF), regardless of the concentration of GGF-2 in the growth medium.
However, GGF-2 alone increased the expression of Krox20, the main transcription factor involved in myelination, relative to those cells treated with all stimulating factors.
Cells lacking fibroblast growth factor were unable to maintain a Schwann-like morphology, and those lacking forskolin exhibited a downregulation in BDNF production.
Therefore, it is likely that the synergistic action of multiple growth factors is required to maintain Schwann-like phenotype in differentiated ASCs.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Mortimer, Alice E.& Faroni, Alessandro& Kilic, Mahmut A.& Reid, Adam J.. 2017. Maintenance of a Schwann-Like Phenotype in Differentiated Adipose-Derived Stem Cells Requires the Synergistic Action of Multiple Growth Factors. Stem Cells International،Vol. 2017, no. 2017, pp.1-7.
https://search.emarefa.net/detail/BIM-1200724
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Mortimer, Alice E.…[et al.]. Maintenance of a Schwann-Like Phenotype in Differentiated Adipose-Derived Stem Cells Requires the Synergistic Action of Multiple Growth Factors. Stem Cells International No. 2017 (2017), pp.1-7.
https://search.emarefa.net/detail/BIM-1200724
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Mortimer, Alice E.& Faroni, Alessandro& Kilic, Mahmut A.& Reid, Adam J.. Maintenance of a Schwann-Like Phenotype in Differentiated Adipose-Derived Stem Cells Requires the Synergistic Action of Multiple Growth Factors. Stem Cells International. 2017. Vol. 2017, no. 2017, pp.1-7.
https://search.emarefa.net/detail/BIM-1200724
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1200724
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر