The Effect and Mechanism of KLF7 in the TLR4NF-κBIL-6 Inflammatory Signal Pathway of Adipocytes
المؤلفون المشاركون
Zhang, Jun
Xie, Jianxin
Wang, Cuizhe
Ha, Xiaodan
Zhang, Meixiu
Wu, Jinxiu
Deng, Yuchun
Zhang, Xueting
Wang, Jingzhou
Chen, Keru
Feng, Jiale
Zhu, Jiaojiao
المصدر
العدد
المجلد 2018، العدد 2018 (31 ديسمبر/كانون الأول 2018)، ص ص. 1-12، 12ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2018-11-26
دولة النشر
مصر
عدد الصفحات
12
التخصصات الرئيسية
الملخص EN
Objective.
To investigate the role and possible molecular mechanism of Krüppel-like factor 7 (KLF7) in the TLR4/NF-κB/IL-6 inflammatory signaling pathway activated by free fatty acids (FFA).
Methods.
The mRNA and protein expression levels of KLF7 and the factors of TLR4/NF-κB/IL-6 inflammatory signal pathways were detected by qRT-PCR and Western blotting after cell culture with different concentrations of palmitic acid (PA).
The expression of KLF7 or TLR4 in adipocytes was upregulated or downregulated; after that, the mRNA and protein expression levels of these key factors were detected.
KLF7 expression was downregulated while PA stimulated adipocytes, and then the mRNA and protein expressions of KLF7/p65 and downstream inflammatory cytokine IL-6 were detected.
The luciferase reporter assay was used to determine whether KLF7 had a transcriptional activation effect on IL-6.
Results.
(1) High concentration of PA can promote the expression of TLR4, KLF7, and IL-6 in adipocytes.
(2) TLR4 positively regulates KLF7 expression in adipocytes.
(3) KLF7 positively regulates IL-6 expression in adipocytes.
(4) PA promotes IL-6 expression via KLF7 in adipocytes.
(5) KLF7 has a transcriptional activation on IL-6.
Conclusion.
PA promotes the expression of the inflammatory cytokine IL-6 by activating the TLR4/KLF7/NF-κB inflammatory signaling pathway.
In addition, KLF7 may directly bind to the IL-6 promoter region and thus activate IL-6.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Zhang, Meixiu& Wang, Cuizhe& Wu, Jinxiu& Ha, Xiaodan& Deng, Yuchun& Zhang, Xueting…[et al.]. 2018. The Effect and Mechanism of KLF7 in the TLR4NF-κBIL-6 Inflammatory Signal Pathway of Adipocytes. Mediators of Inflammation،Vol. 2018, no. 2018, pp.1-12.
https://search.emarefa.net/detail/BIM-1203354
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Zhang, Meixiu…[et al.]. The Effect and Mechanism of KLF7 in the TLR4NF-κBIL-6 Inflammatory Signal Pathway of Adipocytes. Mediators of Inflammation No. 2018 (2018), pp.1-12.
https://search.emarefa.net/detail/BIM-1203354
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Zhang, Meixiu& Wang, Cuizhe& Wu, Jinxiu& Ha, Xiaodan& Deng, Yuchun& Zhang, Xueting…[et al.]. The Effect and Mechanism of KLF7 in the TLR4NF-κBIL-6 Inflammatory Signal Pathway of Adipocytes. Mediators of Inflammation. 2018. Vol. 2018, no. 2018, pp.1-12.
https://search.emarefa.net/detail/BIM-1203354
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1203354
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر