“Redox Imaging” to Distinguish Cells with Different Proliferative Indexes: Superoxide, Hydroperoxides, and Their Ratio as Potential Biomarkers
المؤلفون المشاركون
Higashi, Tatsuya
Bakalova, Rumiana
Zhelev, Zhivko
Aoki, Ichio
Georgieva, Ekaterina
Lazarova, Dessislava
Semkova, Severina
Gulubova, Maya
المصدر
Oxidative Medicine and Cellular Longevity
العدد
المجلد 2019، العدد 2019 (31 ديسمبر/كانون الأول 2019)، ص ص. 1-18، 18ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2019-04-08
دولة النشر
مصر
عدد الصفحات
18
التخصصات الرئيسية
الملخص EN
The present study was directed to the development of EPR methodology for distinguishing cells with different proliferative activities, using “redox imaging.” Three nitroxide radicals were used as redox sensors: (a) mito-TEMPO—cell-penetrating and localized mainly in the mitochondria; (b) methoxy-TEMPO—cell-penetrating and randomly distributed between the cytoplasm and the intracellular organelles; and (c) carboxy-PROXYL—nonpenetrating in living cells and evenly distributed in the extracellular environment.
The experiments were conducted on eleven cell lines with different proliferative activities and oxidative capacities, confirmed by conventional analytical tests.
The data suggest that cancer cells and noncancer cells are characterized by a completely different redox status.
This can be analyzed by EPR spectroscopy using mito-TEMPO and methoxy-TEMPO, but not carboxy-PROXYL.
The correlation analysis shows that the EPR signal intensity of mito-TEMPO in cell suspensions is closely related to the superoxide level.
The described methodology allows the detection of overproduction of superoxide in living cells and their identification based on the intracellular redox status.
The experimental data provide evidences about the role of superoxide and hydroperoxides in cell proliferation and malignancy.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Zhelev, Zhivko& Georgieva, Ekaterina& Lazarova, Dessislava& Semkova, Severina& Aoki, Ichio& Gulubova, Maya…[et al.]. 2019. “Redox Imaging” to Distinguish Cells with Different Proliferative Indexes: Superoxide, Hydroperoxides, and Their Ratio as Potential Biomarkers. Oxidative Medicine and Cellular Longevity،Vol. 2019, no. 2019, pp.1-18.
https://search.emarefa.net/detail/BIM-1204576
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Zhelev, Zhivko…[et al.]. “Redox Imaging” to Distinguish Cells with Different Proliferative Indexes: Superoxide, Hydroperoxides, and Their Ratio as Potential Biomarkers. Oxidative Medicine and Cellular Longevity No. 2019 (2019), pp.1-18.
https://search.emarefa.net/detail/BIM-1204576
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Zhelev, Zhivko& Georgieva, Ekaterina& Lazarova, Dessislava& Semkova, Severina& Aoki, Ichio& Gulubova, Maya…[et al.]. “Redox Imaging” to Distinguish Cells with Different Proliferative Indexes: Superoxide, Hydroperoxides, and Their Ratio as Potential Biomarkers. Oxidative Medicine and Cellular Longevity. 2019. Vol. 2019, no. 2019, pp.1-18.
https://search.emarefa.net/detail/BIM-1204576
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1204576
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر