Oleanolic Acid Decreases IL-1β-Induced Activation of Fibroblast-Like Synoviocytes via the SIRT3-NF-κB Axis in Osteoarthritis
المؤلفون المشاركون
Chen, Zhonggai
Xiong, Yan
Bao, Jiapeng
Yan, Weifeng
Xu, Kai
Chen, Mengyao
Ran, Jisheng
Wu, Lidong
المصدر
Oxidative Medicine and Cellular Longevity
العدد
المجلد 2020، العدد 2020 (31 ديسمبر/كانون الأول 2020)، ص ص. 1-10، 10ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2020-09-29
دولة النشر
مصر
عدد الصفحات
10
التخصصات الرئيسية
الملخص EN
Synovial inflammation is a major pathological feature of osteoarthritis (OA), which is a chronic degenerative joint disease.
Fibroblast-like synoviocytes (FLS), localized in the synovial membrane, are specialized secretory cells.
During OA synovitis, FLS produce chemokines and cytokines that stimulate chondrocytes to secrete inflammatory cytokines and activate matrix metalloproteinases (MMPs) in FLS.
Recent studies have demonstrated that sirtuin 3 (SIRT3) performs as a key regulator in maintaining mitochondrial homeostasis in OA.
This study aims at ascertaining whether SIRT3 is involved in OA synovitis.
The overexpression (OE) and knockdown (KD) of SIRT3 are established by short hairpin RNA (shRNA) and recombinant plasmid in human FLS.
The anti-inflammatory effect of SIRT3 underlying in oleanolic acid- (OLA-) prevented interleukin-1β- (IL-1β-) induced FLS dysfunction is then evaluated in vitro.
Additionally, the molecular mechanisms of SIRT3 are assessed, and the interaction between SIRT3 and NF-κB is investigated.
The data suggested that SIRT3 can be detected in human synovial tissues during OA, and OLA could elevate SIRT3 expression.
OE-SIRT3 and OLA exhibited equal authenticity to repress inflammation and reverse oxidative stress changes in IL-1β-induced human FLS dysfunction.
KD-SIRT3 was found to exacerbate inflammation and oxidative stress changes in human FLS.
Furthermore, it was found that SIRT3 could directly bind with NF-κB, resulting in the suppression of NF-κB activation induced by IL-1β in human FLS, which then repressed synovial inflammation in OA.
In general, the activation of SIRT3 by OLA inhibited synovial inflammation by suppressing the NF-κB signal pathway in FLS, and this suggested that SIRT3 is a potential target for OA synovitis therapy.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Bao, Jiapeng& Yan, Weifeng& Xu, Kai& Chen, Mengyao& Chen, Zhonggai& Ran, Jisheng…[et al.]. 2020. Oleanolic Acid Decreases IL-1β-Induced Activation of Fibroblast-Like Synoviocytes via the SIRT3-NF-κB Axis in Osteoarthritis. Oxidative Medicine and Cellular Longevity،Vol. 2020, no. 2020, pp.1-10.
https://search.emarefa.net/detail/BIM-1205387
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Bao, Jiapeng…[et al.]. Oleanolic Acid Decreases IL-1β-Induced Activation of Fibroblast-Like Synoviocytes via the SIRT3-NF-κB Axis in Osteoarthritis. Oxidative Medicine and Cellular Longevity No. 2020 (2020), pp.1-10.
https://search.emarefa.net/detail/BIM-1205387
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Bao, Jiapeng& Yan, Weifeng& Xu, Kai& Chen, Mengyao& Chen, Zhonggai& Ran, Jisheng…[et al.]. Oleanolic Acid Decreases IL-1β-Induced Activation of Fibroblast-Like Synoviocytes via the SIRT3-NF-κB Axis in Osteoarthritis. Oxidative Medicine and Cellular Longevity. 2020. Vol. 2020, no. 2020, pp.1-10.
https://search.emarefa.net/detail/BIM-1205387
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1205387
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر