The Roles of Skin Fibroblasts at Local Acupoints in Chrono-Acupuncture
المؤلفون المشاركون
Cai, Ding-Jun
Zhou, Qizhi
Liu, Nannan
Zhao, Zhengyu
Zhang, Xiaohong
Wang, Yu
Huang, Shiqi
المصدر
العدد
المجلد 2020، العدد 2020 (31 ديسمبر/كانون الأول 2020)، ص ص. 1-10، 10ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2020-03-26
دولة النشر
مصر
عدد الصفحات
10
التخصصات الرئيسية
الملخص EN
Objective.
The aim of this study was to demonstrate the peripheral mechanisms of chrono-acupuncture by observing acupuncture at different time points affecting relative proteins to regulate the cytoskeleton of fibroblasts differently.
Methods.
A total of 108 male SD rats (180–220 g) that have basic pain threshold within 3–10 s were selected and randomly divided into group A (n = 72) and control group (n = 36).
After the succession of modeling with CFA injection, the rats in group A were randomly divided into model group and acupuncture group, each group containing 36 rats.
Then according to the different treatment time, each group was randomly classified into 6 subgroups (ZT0, ZT4, ZT8, ZT12, ZT16, and ZT20), each subgroup containing 6 rats (n = 6).
On the second day of successful modeling, the rats in the acupuncture group received acupuncture treatment at the corresponding time point, while the control group and the model group were only tied up at the corresponding time point without any treatments.
Methods of operation: use 0.5-inch needles, puncture the rats’ “Zusanli” on the affected limb, with Twirling manipulation for a minute after every five minutes; the treatment lasts thirty minutes in total.
After 7 days of treatments, the skin and subcutaneous tissue of rats’ acupoint area of “Zusanli” on the affected limb were taken and then stained by immunofluorescence double staining method to observe the expression of the fibroblast cytoskeleton F-actin and β-tubulin under the LSCM while using western blot to observe the expression of P38MAPK/P-P38MAPK.
Results.
The expression of the cytoskeleton F-actin and β-tubulin at acupoint area in the acupuncture group was significantly higher than that in the control and model group.
The effect of acupuncture on the restructure of the fibroblast cytoskeleton is different at different time points, the most effective time point was at ZT12 while the least at ZT16.
Acupuncture can decrease the high expression of P-P38MAPK/P38MAPK in the model group, and the effect has time differences.
The expression of P-P38MAPK/P38MAPK increased more significantly at ZT16 than ZT12.
Conclusion.
The remodeling difference of fibroblast cytoskeleton after receiving acupuncture treatment could be one of the peripheral bases of the chrono-acupuncture.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Liu, Nannan& Zhao, Zhengyu& Zhou, Qizhi& Zhang, Xiaohong& Wang, Yu& Huang, Shiqi…[et al.]. 2020. The Roles of Skin Fibroblasts at Local Acupoints in Chrono-Acupuncture. Pain Research and Management،Vol. 2020, no. 2020, pp.1-10.
https://search.emarefa.net/detail/BIM-1206780
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Liu, Nannan…[et al.]. The Roles of Skin Fibroblasts at Local Acupoints in Chrono-Acupuncture. Pain Research and Management No. 2020 (2020), pp.1-10.
https://search.emarefa.net/detail/BIM-1206780
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Liu, Nannan& Zhao, Zhengyu& Zhou, Qizhi& Zhang, Xiaohong& Wang, Yu& Huang, Shiqi…[et al.]. The Roles of Skin Fibroblasts at Local Acupoints in Chrono-Acupuncture. Pain Research and Management. 2020. Vol. 2020, no. 2020, pp.1-10.
https://search.emarefa.net/detail/BIM-1206780
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1206780
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر