Nucleus Pulposus Cell Conditioned Medium Promotes Mesenchymal Stem Cell Differentiation into Nucleus Pulposus-Like Cells under Hypoxic Conditions
المؤلفون المشاركون
Zhang, Cong
Chen, Lu
Sinkemani, Arjun
Xie, Zhiyang
Wu, Xiaotao
Wang, Feng
المصدر
العدد
المجلد 2020، العدد 2020 (31 ديسمبر/كانون الأول 2020)، ص ص. 1-24، 24ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2020-12-23
دولة النشر
مصر
عدد الصفحات
24
الملخص EN
Low back pain (LBP) is a major physical and socioeconomic challenge worldwide.
Nucleus pulposus (NP) is directly associated with LBP due to intervertebral disc (IVD) degeneration.
IVD degeneration is mainly caused by structural and matrix-related changes within the IVD occurring during aging and degeneration.
Mesenchymal stem cells (MSCs) can differentiate into multiple mesenchymal lineages under specific stimulatory conditions.
This study is aimed at evaluating the effectiveness of the nucleus pulposus cell (NPC) conditioned medium for promoting the expression of MSCs and at confirming the expression of healthy NP phenotypic markers recently recommended by the Spine Research Interest Group.
Expression was investigated using quantitative polymerase chain reaction (qPCR) and western blotting under normoxic and hypoxic conditions.
qPCR and western blotting demonstrated significant upregulation of NP marker expression in MSCs cultured under hypoxic conditions and treated with the 50% or 100% NPC conditioned medium, compared with those cultured under normoxic conditions.
Upregulation was highest in the presence of the 100% NPC conditioned medium compared with the control group (aggrecan, p<0.01; brachyury, p<0.05; collagen II, p<0.001; KRT8, p<0.01; KRT19, p<0.001; and Shh, p<0.01).
The expression levels of genes in MSCs treated with the 50% NPC conditioned medium also showed upregulation compared with the control group (collagen II, p<0.05; KRT8, p<0.05; and KRT19, p<0.01).
These findings suggested that the NPC conditioned medium stimulated MSC differentiation into an NP-like phenotype with distinct characteristics.
The results could inform strategies for IVD regeneration.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Sinkemani, Arjun& Wang, Feng& Xie, Zhiyang& Chen, Lu& Zhang, Cong& Wu, Xiaotao. 2020. Nucleus Pulposus Cell Conditioned Medium Promotes Mesenchymal Stem Cell Differentiation into Nucleus Pulposus-Like Cells under Hypoxic Conditions. Stem Cells International،Vol. 2020, no. 2020, pp.1-24.
https://search.emarefa.net/detail/BIM-1208050
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Sinkemani, Arjun…[et al.]. Nucleus Pulposus Cell Conditioned Medium Promotes Mesenchymal Stem Cell Differentiation into Nucleus Pulposus-Like Cells under Hypoxic Conditions. Stem Cells International No. 2020 (2020), pp.1-24.
https://search.emarefa.net/detail/BIM-1208050
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Sinkemani, Arjun& Wang, Feng& Xie, Zhiyang& Chen, Lu& Zhang, Cong& Wu, Xiaotao. Nucleus Pulposus Cell Conditioned Medium Promotes Mesenchymal Stem Cell Differentiation into Nucleus Pulposus-Like Cells under Hypoxic Conditions. Stem Cells International. 2020. Vol. 2020, no. 2020, pp.1-24.
https://search.emarefa.net/detail/BIM-1208050
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1208050
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر