Potential Role of the Resident Mesenchymal Stem-Like Cells in Renal Fibrogenesis after Ureteral Obstruction
المؤلفون المشاركون
Li, Tao-Sheng
Peng, Yong-Hua
Xiao, Jie
Yan, Chen
Luo, Lan
المصدر
العدد
المجلد 2019، العدد 2019 (31 ديسمبر/كانون الأول 2019)، ص ص. 1-11، 11ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2019-03-05
دولة النشر
مصر
عدد الصفحات
11
الملخص EN
The mechanisms of renal fibrogenesis after ureteral obstruction remain unclear.
We tried to primarily expand mesenchymal stem cells from renal tissues and then investigated their role in fibrogenesis after ureteral obstruction.
Unilateral ureteral obstruction was induced by ligating the left ureteral duct of adult C57BL/6 mice.
We collected the kidneys for experiments at 2, 7, and 14 days after operation.
Histological analysis showed obviously fibrotic changes in the left kidney at 7 days and further increased at 14 days after ureteral obstruction.
To expand mesenchymal stem cells, we minced the renal tissues into small explants (about 1 mm3) and cultured onto 10 cm dishes.
Interestingly, the outgrowth of cells was observed significantly earlier from the explants of the obstructed left kidney than that of the unobstructed right kidney.
These expanded cells showed the potency of adipogenic, osteogenic, and chondrogenic differentiations and positively expressed with CD44 and partly expressed with CD90, CD105, and CD106, but negatively expressed with CD34, CD45, and FSP1, suggesting the phenotype of mesenchymal stem-like cells (MSLCs).
The mouse fibrosis RT2 profiler PCR array showed that many genes were changed over 2-fold in the MSLCs expanded from both kidneys at 2, 7, and 14 days after operation.
Interestingly, profibrotic genes were prevalently enhanced in the left kidney with ureteral obstruction.
Histological analysis also showed obviously infiltration of inflammatory cells in the left kidney at 14 days after operation.
Our data indicate the potential role of resident MSLCs in renal fibrogenesis after ureteral obstruction, but further experiments are required to understand the relevant mechanisms.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Peng, Yong-Hua& Xiao, Jie& Yan, Chen& Luo, Lan& Li, Tao-Sheng. 2019. Potential Role of the Resident Mesenchymal Stem-Like Cells in Renal Fibrogenesis after Ureteral Obstruction. Stem Cells International،Vol. 2019, no. 2019, pp.1-11.
https://search.emarefa.net/detail/BIM-1208498
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Peng, Yong-Hua…[et al.]. Potential Role of the Resident Mesenchymal Stem-Like Cells in Renal Fibrogenesis after Ureteral Obstruction. Stem Cells International No. 2019 (2019), pp.1-11.
https://search.emarefa.net/detail/BIM-1208498
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Peng, Yong-Hua& Xiao, Jie& Yan, Chen& Luo, Lan& Li, Tao-Sheng. Potential Role of the Resident Mesenchymal Stem-Like Cells in Renal Fibrogenesis after Ureteral Obstruction. Stem Cells International. 2019. Vol. 2019, no. 2019, pp.1-11.
https://search.emarefa.net/detail/BIM-1208498
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1208498
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر