Pyridoxal-5′-Phosphate Promotes Immunomodulatory Function of Adipose-Derived Mesenchymal Stem Cells through Indoleamine 2,3-Dioxygenase-1 and TLR4NF-κB Pathway
المؤلفون المشاركون
Li, Dong
Ju, Xiuli
Li, Cong
Huang, Jinxian
Zhu, Huasu
Shi, Qing
المصدر
العدد
المجلد 2019، العدد 2019 (31 ديسمبر/كانون الأول 2019)، ص ص. 1-15، 15ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2019-11-25
دولة النشر
مصر
عدد الصفحات
15
الملخص EN
Adipose-derived mesenchymal stem cells (A-MSCs) are promising cellular therapies for the treatment of immune-mediated diseases.
Non-gene editing technologies can improve the immune regulatory function of A-MSCs.
Our preliminary experiments revealed that an active form of vitamin B6—pyridoxal-5′-phosphate (PLP)—plays an important role in regulating gene expression and cytokine secretion in A-MSCs in vivo.
To further clarify the effect of PLP on receptors and cytokines related to the immune regulatory function of A-MSCs, a series of experiments were designed to verify the relationships between PLP and A-MSCs in vitro.
Initially, A-MSCs were obtained, and cytokine secretion and the expression of IDO1, NF-κB, and Toll-like receptors in PLP-stimulated A-MSCs were evaluated.
In addition, coculture was used to detect A-MSCs-mediated apoptosis of CD3+CD8+ T lymphocytes.
These results showed that A-MSCs stimulated with PLP were highly proliferative, consistent with their pluripotent capacity.
Further, the surface receptors TLR3, TLR4, IDO1, and NF-κB were upregulated, while TLR6 was downregulated.
Concurrently, A-MSCs preconditioned with PLP had the greatest inhibitory effect on CD3+CD8+ T lymphocyte proliferation, indicating that PLP altered the immune regulatory function of A-MSCs through the regulation of TLRs and IDO1 expression.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Li, Cong& Huang, Jinxian& Zhu, Huasu& Shi, Qing& Li, Dong& Ju, Xiuli. 2019. Pyridoxal-5′-Phosphate Promotes Immunomodulatory Function of Adipose-Derived Mesenchymal Stem Cells through Indoleamine 2,3-Dioxygenase-1 and TLR4NF-κB Pathway. Stem Cells International،Vol. 2019, no. 2019, pp.1-15.
https://search.emarefa.net/detail/BIM-1208607
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Li, Cong…[et al.]. Pyridoxal-5′-Phosphate Promotes Immunomodulatory Function of Adipose-Derived Mesenchymal Stem Cells through Indoleamine 2,3-Dioxygenase-1 and TLR4NF-κB Pathway. Stem Cells International No. 2019 (2019), pp.1-15.
https://search.emarefa.net/detail/BIM-1208607
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Li, Cong& Huang, Jinxian& Zhu, Huasu& Shi, Qing& Li, Dong& Ju, Xiuli. Pyridoxal-5′-Phosphate Promotes Immunomodulatory Function of Adipose-Derived Mesenchymal Stem Cells through Indoleamine 2,3-Dioxygenase-1 and TLR4NF-κB Pathway. Stem Cells International. 2019. Vol. 2019, no. 2019, pp.1-15.
https://search.emarefa.net/detail/BIM-1208607
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1208607
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر