Development of Bifunctional Three-Dimensional Cysts from Chemically Induced Liver Progenitors
المؤلفون المشاركون
Huang, Yu
Sakai, Yusuke
Hara, Takanobu
Katsuda, Takeshi
Adachi, Tomohiko
Hidaka, Masaaki
Gu, Wei-Li
Eguchi, Susumu
Ochiya, Takahiro
المصدر
العدد
المجلد 2019، العدد 2019 (31 ديسمبر/كانون الأول 2019)، ص ص. 1-13، 13ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2019-09-03
دولة النشر
مصر
عدد الصفحات
13
الملخص EN
Chemically induced liver progenitors (CLiPs) have promising applications in liver regenerative medicine.
Three-dimensional (3D) structures generated from liver progenitor cells possess wide applications in cell transplantation, disease model, and drug testing.
Here, we report on the spontaneous formation of 3D cystic structures comprising maturing rat CLiPs on gelatin-coated dishes.
Our 3D cysts contained Alb+/+CK19+/− and Ck19+/+Alb+/− cells.
These cell types gradually diverged into specialized mature cells, as demonstrated by the expression of mature biliary markers (Cftr, Ae2, and Aqp1) and hepatic markers (Alb and Mrp2).
The 3D cysts also expressed functional multidrug resistance protein 1 (Mdr1), as indicated by epithelial efflux of rhodamine.
Furthermore, we observed bile canaliculi functions between hepatocytes and cholyl-lysyl-fluorescein extrusions, indicating that the functional characteristics of 3D cysts and active bile salt export pump (Bsep) transporters were intact.
Thus, our study revealed a natural characteristic of rat CLiPs to spontaneously form 3D cystic structures accompanied with cell maturation in vitro, offering a platform for studies of liver development and drug screening.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Huang, Yu& Sakai, Yusuke& Hara, Takanobu& Katsuda, Takeshi& Ochiya, Takahiro& Adachi, Tomohiko…[et al.]. 2019. Development of Bifunctional Three-Dimensional Cysts from Chemically Induced Liver Progenitors. Stem Cells International،Vol. 2019, no. 2019, pp.1-13.
https://search.emarefa.net/detail/BIM-1208770
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Huang, Yu…[et al.]. Development of Bifunctional Three-Dimensional Cysts from Chemically Induced Liver Progenitors. Stem Cells International No. 2019 (2019), pp.1-13.
https://search.emarefa.net/detail/BIM-1208770
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Huang, Yu& Sakai, Yusuke& Hara, Takanobu& Katsuda, Takeshi& Ochiya, Takahiro& Adachi, Tomohiko…[et al.]. Development of Bifunctional Three-Dimensional Cysts from Chemically Induced Liver Progenitors. Stem Cells International. 2019. Vol. 2019, no. 2019, pp.1-13.
https://search.emarefa.net/detail/BIM-1208770
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1208770
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر