Serum-Free Culture of Human Mesenchymal Stem Cell Aggregates in Suspension Bioreactors for Tissue Engineering Applications
المؤلفون المشاركون
Sen, Arindom
Allen, Leah M.
Matyas, John
Ungrin, Mark
Hart, David A.
المصدر
العدد
المجلد 2019، العدد 2019 (31 ديسمبر/كانون الأول 2019)، ص ص. 1-18، 18ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2019-11-07
دولة النشر
مصر
عدد الصفحات
18
الملخص EN
Mesenchymal stem cells (MSCs) have the capacity to differentiate towards bone, fat, and cartilage lineages.
The most widely used culture and differentiation protocols for MSCs are currently limited by their use of serum-containing media and small-scale static culture vessels.
Suspension bioreactors have multiple advantages over static culture vessels (e.g., scalability, control, and mechanical forces).
This study sought to compare the formation and culture of 3D aggregates of human synovial fluid MSCs within suspension bioreactors and static microwell plates.
It also sought to elucidate the benefits of these techniques in terms of productivity, cell number, and ability to generate aggregates containing extracellular matrix deposition.
MSCs in serum-free medium were either (1) inoculated as single cells into suspension bioreactors, (2) aggregated using static microwell plates prior to being inoculated in the bioreactor environment, or (3) aggregated using microwell plates and kept in the static environment.
Preformed aggregates that were size-controlled at inoculation had a greater tendency to form large, irregular super aggregates after a few days of suspension culture.
The single MSCs inoculated into suspension bioreactors formed a more uniform population of smaller aggregates after a definite culture period of 8 days.
Both techniques showed initial deposition of extracellular matrix within the aggregates.
When the relationship between aggregate size and ECM deposition was investigated in static culture, midsized aggregates (100-300 cells/aggregate) were found to most consistently maximize sGAG and collagen productivity.
Thus, this study presents a 3D tissue culture method, which avoids the clinical drawbacks of serum-containing medium that can easily be scaled for tissue culture applications.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Allen, Leah M.& Matyas, John& Ungrin, Mark& Hart, David A.& Sen, Arindom. 2019. Serum-Free Culture of Human Mesenchymal Stem Cell Aggregates in Suspension Bioreactors for Tissue Engineering Applications. Stem Cells International،Vol. 2019, no. 2019, pp.1-18.
https://search.emarefa.net/detail/BIM-1208869
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Allen, Leah M.…[et al.]. Serum-Free Culture of Human Mesenchymal Stem Cell Aggregates in Suspension Bioreactors for Tissue Engineering Applications. Stem Cells International No. 2019 (2019), pp.1-18.
https://search.emarefa.net/detail/BIM-1208869
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Allen, Leah M.& Matyas, John& Ungrin, Mark& Hart, David A.& Sen, Arindom. Serum-Free Culture of Human Mesenchymal Stem Cell Aggregates in Suspension Bioreactors for Tissue Engineering Applications. Stem Cells International. 2019. Vol. 2019, no. 2019, pp.1-18.
https://search.emarefa.net/detail/BIM-1208869
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1208869
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر