A Liquid Chromatography with Tandem Mass Spectrometry-Based Proteomic Analysis of Primary Cultured Cells and Subcultured Cells Using Mouse Adipose-Derived Mesenchymal Stem Cells
المؤلفون المشاركون
Fujita, Jiro
Noguchi, Hirofumi
Saitoh, Issei
Nakashima, Yoshiki
Nahar, Saifun
Miyagi-Shiohira, Chika
Kinjo, Takao
Kobayashi, Naoya
Watanabe, Masami
المصدر
العدد
المجلد 2019، العدد 2019 (31 ديسمبر/كانون الأول 2019)، ص ص. 1-97، 97ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2019-01-10
دولة النشر
مصر
عدد الصفحات
97
الملخص EN
Adipose-derived mesenchymal stem cells (MSC-ATs) are representative cell sources for cell therapy.
However, how cell stress resulting from passage influences the MSC-AT protein expression has been unclear.
In this study, a protein expression analysis was performed by liquid chromatography with tandem mass spectrometry (LC-MS/MS) using mouse primary cultured cells (P0) and cells passaged three times (P3) as samples.
A total of 256 proteins were classified as cellular process-related proteins, while 179 were classified as metabolic process-related proteins in P0.
These were considered to be adaptive responses of the cells to an in vitro environment.
However, seven proteins of growth were identified (Csf1, App, Adam15, Alcam, Tbl1xr1, Ninj1, and Sbds) in P0.
In addition, four proteins of antioxidant activity were also identified (Srxn1, Txndc17, Fam213b, and Apoe) in P0.
We identified 1139 proteins expressed in both P0 and P3 cells that had their expression decreased to 69.4% in P3 cells compared with P0 cells, but 1139 proteins are very likely proteins that are derived from MSC-AT.
The function of MSC-ATs was maintained after three passages.
However, the LC-MS/MS analysis data showed that the protein expression was degraded after three passages.
MSC-ATs retained about 70% of their protein expression ability in P3 cells.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Nakashima, Yoshiki& Nahar, Saifun& Miyagi-Shiohira, Chika& Kinjo, Takao& Kobayashi, Naoya& Saitoh, Issei…[et al.]. 2019. A Liquid Chromatography with Tandem Mass Spectrometry-Based Proteomic Analysis of Primary Cultured Cells and Subcultured Cells Using Mouse Adipose-Derived Mesenchymal Stem Cells. Stem Cells International،Vol. 2019, no. 2019, pp.1-97.
https://search.emarefa.net/detail/BIM-1209523
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Nakashima, Yoshiki…[et al.]. A Liquid Chromatography with Tandem Mass Spectrometry-Based Proteomic Analysis of Primary Cultured Cells and Subcultured Cells Using Mouse Adipose-Derived Mesenchymal Stem Cells. Stem Cells International No. 2019 (2019), pp.1-97.
https://search.emarefa.net/detail/BIM-1209523
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Nakashima, Yoshiki& Nahar, Saifun& Miyagi-Shiohira, Chika& Kinjo, Takao& Kobayashi, Naoya& Saitoh, Issei…[et al.]. A Liquid Chromatography with Tandem Mass Spectrometry-Based Proteomic Analysis of Primary Cultured Cells and Subcultured Cells Using Mouse Adipose-Derived Mesenchymal Stem Cells. Stem Cells International. 2019. Vol. 2019, no. 2019, pp.1-97.
https://search.emarefa.net/detail/BIM-1209523
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1209523
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر