Reprogrammed Cells Display Distinct Proteomic Signatures Associated with Colony Morphology Variability
المؤلفون المشاركون
Ræder, Helge
Bjørlykke, Yngvild
Søviknes, Anne M.
Hoareau, Laurence
Vethe, Heidrun
Mathisen, Andreas F.
Chera, Simona
Vaudel, Marc
Ghila, Luiza M.
المصدر
العدد
المجلد 2019، العدد 2019 (31 ديسمبر/كانون الأول 2019)، ص ص. 1-16، 16ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2019-11-18
دولة النشر
مصر
عدد الصفحات
16
الملخص EN
Human induced pluripotent stem cells (hiPSCs) are of high interest because they can be differentiated into a vast range of different cell types.
Ideally, reprogrammed cells should sustain long-term culturing in an undifferentiated state.
However, some reprogrammed cell lines represent an unstable state by spontaneously differentiating and changing their cellular phenotype and colony morphology.
This phenomenon is not fully understood, and no method is available to predict it reliably.
In this study, we analyzed and compared the proteome landscape of 20 reprogrammed cell lines classified as stable and unstable based on long-term colony morphology.
We identified distinct proteomic signatures associated with stable colony morphology and with unstable colony morphology, although the typical pluripotency markers (POU5F1, SOX2) were present with both morphologies.
Notably, epithelial to mesenchymal transition (EMT) protein markers were associated with unstable colony morphology, and the transforming growth factor beta (TGFB) signalling pathway was predicted as one of the main regulator pathways involved in this process.
Furthermore, we identified specific proteins that separated the stable from the unstable state.
Finally, we assessed both spontaneous embryonic body (EB) formation and directed differentiation and showed that reprogrammed lines with an unstable colony morphology had reduced differentiation capacity.
To conclude, we found that different defined patterns of colony morphology in reprogrammed cells were associated with distinct proteomic profiles and different outcomes in differentiation capacity.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Bjørlykke, Yngvild& Søviknes, Anne M.& Hoareau, Laurence& Vethe, Heidrun& Mathisen, Andreas F.& Chera, Simona…[et al.]. 2019. Reprogrammed Cells Display Distinct Proteomic Signatures Associated with Colony Morphology Variability. Stem Cells International،Vol. 2019, no. 2019, pp.1-16.
https://search.emarefa.net/detail/BIM-1209627
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Bjørlykke, Yngvild…[et al.]. Reprogrammed Cells Display Distinct Proteomic Signatures Associated with Colony Morphology Variability. Stem Cells International No. 2019 (2019), pp.1-16.
https://search.emarefa.net/detail/BIM-1209627
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Bjørlykke, Yngvild& Søviknes, Anne M.& Hoareau, Laurence& Vethe, Heidrun& Mathisen, Andreas F.& Chera, Simona…[et al.]. Reprogrammed Cells Display Distinct Proteomic Signatures Associated with Colony Morphology Variability. Stem Cells International. 2019. Vol. 2019, no. 2019, pp.1-16.
https://search.emarefa.net/detail/BIM-1209627
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1209627
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر