β-Fructofuranosidase and β –D-Fructosyltransferase from New Aspergillus carbonarius PC-4 Strain Isolated from Canned Peach Syrup: Effect of Carbon and Nitrogen Sources on Enzyme Production

المؤلفون المشاركون

de Almeida, Alex Fernando
Nascimento, Gustavo Carvalho do
Batista, Ryhára Dias
Santos, Claudia Cristina Auler do Amaral
Silva, Ezequiel Marcelino da
de Paula, Fabrício Coutinho
Mendes, Danylo Bezerra
de Oliveira, Deyla Paula

المصدر

The Scientific World Journal

العدد

المجلد 2019، العدد 2019 (31 ديسمبر/كانون الأول 2019)، ص ص. 1-13، 13ص.

الناشر

Hindawi Publishing Corporation

تاريخ النشر

2019-01-08

دولة النشر

مصر

عدد الصفحات

13

التخصصات الرئيسية

الطب البشري
تكنولوجيا المعلومات وعلم الحاسوب

الملخص EN

β-fructofuranosidase (invertase) and β-D-fructosyltransferase (FTase) are enzymes used in industrial processes to hydrolyze sucrose aiming to produce inverted sugar syrup or fructooligosaccharides.

In this work, a black Aspergillus sp.

PC-4 was selected among six filamentous fungi isolated from canned peach syrup which were initially screened for invertase production.

Cultivations with pure carbon sources showed that invertase and FTase were produced from glucose and sucrose, but high levels were also obtained from raffinose and inulin.

Pineapple crown was the best complex carbon source for invertase (6.71 U/mL after 3 days of cultivation) and FTase production (14.60 U/mL after 5 days of cultivation).

Yeast extract and ammonium chloride nitrogen sources provided higher production of invertase (6.80 U/mL and 6.30 U/mL, respectively), whereas ammonium nitrate and soybean protein were the best nitrogen sources for FTase production (24.00 U/mL and 24.90 U/mL, respectively).

Fermentation parameters for invertase using yeast extract were YP/S = 536.85 U/g and PP = 1.49 U/g/h.

FTase production showed values of YP/S = 2,627.93 U/g and PP = 4.4 U/h using soybean protein.

The screening for best culture conditions showed an increase of invertase production values by 5.10-fold after 96 h cultivation compared to initial experiments (fungi bioprospection), while FTase production increased by 14.60-fold (44.40 U/mL) after 168 h cultivation.

A.

carbonarius PC-4 is a new promising strain for invertase and FTase production from low cost carbon sources, whose synthesized enzymes are suitable for the production of inverted sugar, fructose syrups, and fructooligosaccharides.

نمط استشهاد جمعية علماء النفس الأمريكية (APA)

Nascimento, Gustavo Carvalho do& Batista, Ryhára Dias& Santos, Claudia Cristina Auler do Amaral& Silva, Ezequiel Marcelino da& de Paula, Fabrício Coutinho& Mendes, Danylo Bezerra…[et al.]. 2019. β-Fructofuranosidase and β –D-Fructosyltransferase from New Aspergillus carbonarius PC-4 Strain Isolated from Canned Peach Syrup: Effect of Carbon and Nitrogen Sources on Enzyme Production. The Scientific World Journal،Vol. 2019, no. 2019, pp.1-13.
https://search.emarefa.net/detail/BIM-1211886

نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)

Nascimento, Gustavo Carvalho do…[et al.]. β-Fructofuranosidase and β –D-Fructosyltransferase from New Aspergillus carbonarius PC-4 Strain Isolated from Canned Peach Syrup: Effect of Carbon and Nitrogen Sources on Enzyme Production. The Scientific World Journal No. 2019 (2019), pp.1-13.
https://search.emarefa.net/detail/BIM-1211886

نمط استشهاد الجمعية الطبية الأمريكية (AMA)

Nascimento, Gustavo Carvalho do& Batista, Ryhára Dias& Santos, Claudia Cristina Auler do Amaral& Silva, Ezequiel Marcelino da& de Paula, Fabrício Coutinho& Mendes, Danylo Bezerra…[et al.]. β-Fructofuranosidase and β –D-Fructosyltransferase from New Aspergillus carbonarius PC-4 Strain Isolated from Canned Peach Syrup: Effect of Carbon and Nitrogen Sources on Enzyme Production. The Scientific World Journal. 2019. Vol. 2019, no. 2019, pp.1-13.
https://search.emarefa.net/detail/BIM-1211886

نوع البيانات

مقالات

لغة النص

الإنجليزية

الملاحظات

Includes bibliographical references

رقم السجل

BIM-1211886