تأثير التلقيح الحيوي ببكتريا Klebsiella Pneumoniea و فطريات المايكورايزا Glomus Mosseae و دور حامض الهيومك و المبيد الحيوي Trichoderma Harzianum في حاصل و نسبة الزيت في الحبة السوداء Nigella Sativa

الملخص EN

A global experiment was conducted in the greenhouses of the College of Agriculture and Forestry / University of Mosul using a mixture of soil sample taken from the Rashidiya region in Nineveh Governorate, northern Iraq, and placed in 6-kilogram plastic anvils with six transactions and three replications of black seed (black seed) seeded during the winter season 2019.

The soil was inoculated with K.

pneumoniea bacteria, obtained from the Media Center in Erbil and isolated on MacConKey agar, as well as from the Department of Life Sciences / College of Science, University of Mosul.

The fungicide T.

harzianum was tested in three levels, and the ability of K.

pneumoniea to stabilize atmospheric nitrogen on nitrogen-free broth (NF) broth was tested.

The results showed the efficiency of K.

pneumoniea in fixing atmospheric nitrogen by relying on measuring the efficacy of the nitrogenase enzyme by Nessler's reagent.

The resistance of bacteria to the fungicide T.

harzianum at the second and third levels (0.5, 0.250 g / l), and the study also showed a significant effect, as the treatment of inoculation with microscopic and Klebsiella bacteria and the addition of humic acid gave a significant increase of 68 cm, as well as the interaction between them gave the highest values of 75 cm compared to the treatment The comparison was 37 cm, as well as the presence of significant differences in the weight of the yield, as the organic fertilizer treatment achieved a level of 100% with the interaction between the two types of biological fertilizer, which gave the highest rate of (9.73 g) compared to the comparison treatment, which gave the lowest rate of (1.03 g).

The results also gave Significant effect on the quality of the oil yield in all treatments and in the overlap between them and the overlap exceeded by giving it the highest rate of the characteristic, which reached 41.81% , and with the addition of the highest level of organic fertilizer compared with no addition of poison.

D.

Organic and the comparison treatment gave the lowest oil content rate (39.66% and 22.53% ) with an increase rate (2.15% and 19.38% ).

The statistical design CRD was used and the results were analyzed using Duncan for means.