In vitro buffalo embryo production under heat stress : role of granulosa cells co-culture as a monolayer
المؤلفون المشاركون
Ghanim, Nasir
Samy, Romysa
Barkawi, A. H.
Fahim, Marwah Said
المصدر
Annals of Agricultural Science, Moshtohor
الناشر
تاريخ النشر
2021-12-31
دولة النشر
مصر
عدد الصفحات
1
التخصصات الرئيسية
الموضوعات
الملخص الإنجليزي
Effect ofheat shock and co-culture of monolayer granulosa cells (GCs) on in vitroembryo developmental competence in Egyptian buffalo was the aim of the present study.
Cumulus oocytes complexes (COCs) were collected from ovaries (n= 448) of cyclicslaughtered buffalo cows.
Good quality immature oocytes (n= 1512) were subjected to in vitro maturation and fertilization.
Post 18-22 h of fertilization, presumptive embryos were randomly assigned into four groups: (G1) No heat shock (38.5OC), (G2) Heat shock (40.5°C) and (G3) Co-culture with GCs monolayer and heat shock or (G4) Co-culture with GCs monolayer and no heat shock.
Heat-shockedembryos were exposed to temperature of40.5°Cfor the first two hours of culture before exposing to 38.5°C afterward up to the end of culture perio d (8 days).
The data were analyzed by General LinearUnivariate model using SPSS.
Oocyte expansionrate was 90.8±1.1% .
This percentage is higher than nuclear maturation rate (oocytes at Telophase and Metaphase II) as detected byaceto-orcein staining by about 17% .
Cleavage rate as recorded at day 3 post fertilization (day 0) was higher (p≤0.05) for G1 and G4 (71.1±10.5% and 70.5±7.9 with GCs, respectively).
On the other hand, cleavage rate was dramatically decreased (P ≤0.05) for embryos of G2 (43.7±7.0% ) compared with that of G3 (80.2±7.0% ).
In addition, embryos of G3showed approximately the same rate ofdeveloped embryos(Morula and blastocyst stages at day 8 of culture) as of G1 (50.9±5.3 and51.7±7.9% , respectively).
In conclusion, using co-culture of GCs as a monolayer enhances cleavage rate and subsequent embryo development of buffalo embryos under heat stress condition.
نوع البيانات
أوراق مؤتمرات
رقم السجل
BIM-1251243
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Fahim, Marwah Said& Ghanim, Nasir& Samy, Romysa& Barkawi, A. H.. 2021-12-31. In vitro buffalo embryo production under heat stress : role of granulosa cells co-culture as a monolayer. . Vol. 59, Special issue (2021), pp.103-103.Qalyoubia Egypt : Banha University Faculty of Agriculture.
https://search.emarefa.net/detail/BIM-1251243
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Fahim, Marwah Said…[et al.]. In vitro buffalo embryo production under heat stress : role of granulosa cells co-culture as a monolayer. . Qalyoubia Egypt : Banha University Faculty of Agriculture. 2021-12-31.
https://search.emarefa.net/detail/BIM-1251243
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Fahim, Marwah Said& Ghanim, Nasir& Samy, Romysa& Barkawi, A. H.. In vitro buffalo embryo production under heat stress : role of granulosa cells co-culture as a monolayer. .
https://search.emarefa.net/detail/BIM-1251243
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر