استخدام 2،2-ثنائي البريديل في التقدير الطيفي للأسيتيل سيستين في حالته النقية و في مستحضراته الصيدلانية

الملخص EN

This study includes development of a sensitive spectrophotometric method for the determination of acetylcysteine in aqueous solution.

The method is based on the oxidation of acetylcysteine with a ferric ion, followed by reacting of produced ferrous ion with 2,2-bipyridyl to form a pink complex, which is stable and water-soluble and has the highest absorption at a wavelength of 524 nm.

The limits of Beer's law were in the concentration range of (5-180) µg of acetylcysteine in a final volume of 20 milliliters (0.25-9.0 µg.ml-¹) and the molar absorptivity of 8633.28 L.

mol-¹ .cm¹, Sandell's sensitivity index of 0.0189 µg.cm².

The relative error of -4.28% -4.98 % and relative standard deviation of ± 0.56% - ± 3.75% depending on the concentration level.

The limit of detection limit(LOD) and limit of quantitation (LOQ) were calculated and equal to 0.01837 and 0.06124 µg/ml respectively, no inference was observed in the common pharmaceutical excipients.

The results obtain by proposed method were in good agreement with those obtain from official British pharmacopoeia using t-test (at 95% confidence limit) which indicates that there is no significant differences between them.

The method was successfully applied to determine acetylcysteine in its pharmaceutical preparations.