تقصي الفعالية المثبطة لأنزيم الليباز لعدة أنواع من جنسي الزعرور و العليق في سوريا و عزل و تحديد المركب الفعال

الملخص EN

One of the treatment strategies for Obesity is to inhibit specific digestive enzymes in order to reduce fat absorption in the intestines.

In this study, inhibitory activity against lipase were determined for the methanolic extracts of leaves of two species of the genus Crataegus; monogyna, and azarolus var.

aronia, and three species of the genus Rubus; sanctus, hedycarpus, and collinus.

The plants were collected during spring and autumn seasons from different regions across the Syrian Arab Republic.

The methanolic extract of Rubus sanctus collected in autumn exhibited the highest activity compared to the other extracts.

For a closer look into this plant, the methanolic extract was partitioned into three extracts using water, ethyl acetate, and diethyl ether as solvents.

Due to promising activity of the ethyl acetate extract against lipase with an IC50=107 µg/ml, this extract was further fractionated by means of preparative HPLC.

The structure of the isolated compound that revealed the strongest inhibitory activity toward lipase enzyme (IC50=91 µg/ml) was elucidated using nuclear magnetic resonance (NMR), and mass spectroscopy (MS) techniques.

The structure was identified based on MS and NMR data to be Quercetin, which isolated for the first time from R.sanctus.