سقالة الببتيد هيدروجيل ®Puramatrix تدعم عيوشية الخلايا الشحمية العائدة عن التمايز في المختبر

الملخص EN

Bioscaffold enhances cell adhesion and proliferation, as well as, diffusion of growth factors and nutrients, and thus improve proliferation and differentiation of stem cells during tissue regeneration.

Peptide hydrogel nanofiber Puramatrix® (PM) presents a unique gel scaffold to support cell proliferation and creates microenviroment mimic the extracellular matrix for tissue engineering.

This study aimed to evaluate the viability of dedifferentiated fat cells DFAT harvested from fat tissue of dog into synthesized peptide hydrogel puramatrix® (an in vitro study).

Dedifferentiated fat cells DFAT were isolated from dog fat tissue by collagenase-digestion and later centrifuging.

The DFAT in the 2rd Passage were seeded into 96 well plates.

After 24 hours, three concentrations of PM (0.15, 0.25, 0.5%) were added to experimental wells.

Two plates were adopted : 48 hours, 7 days.

The DFAT viability into PM scaffold was evaluated by MTT assay.

The results showed no significant difference between the three PM concentrations within and with control group after 48 hours of DFAT cell seeding into PM scaffold (P=0.563).

On the other hand, after 7 days, the three PM concentrations have significantly improved DFAT cell viability, compared with control group (P<0.001).

However, the two PM (0.15, 0.25%) have increased DFAT cell viability compared with 0.5% after 7 days of culturing (P<0.05).