دراسة مرجعية لتقنية CRISPR-Cas، و تطبيقاتها في التعديل الجيني و معالجة الأمراض الوراثية، و التقانة الحيوية الصناعية

الملخص EN

This review aims to overview CRISPR-Cas technology, and its applications in gene editing and inherited diseases treatment.

Moreover, the study covers this technology uses in industrial biotechnology, either in the improvement of primary and secondary metabolite from bacteria and yeasts, or in crops development.

This technology is considered a revolution in gene editing world, due to its versatility and capability of targeting virtually any gene fragment from any organism, which makes it has a wide range of applications, from medicine and pharmacy to agriculture and industry.

This study focuses on the mechanism of CRISPR- Cas, as natural adaptive immunity system in prokaryotes, the exploiting of it in gene editing and uses of this editing as a useful tool in inherited diseases treatment and the development of industrial bacterial, yeast and plant strains.