دراسة السمية الجينية التي تسببها صبغات الشعر الدائمة الحاوية على بارافينيلين دي أمين في الزجاج

الملخص EN

ρ-Phenylenediamine (PPD) is an aromatic amine that contains an amine group in para position in its chemical structure and it's one of the known compounds used in permanent hair dyes as a primary intermediate with another component called coupler to format reaction products which have amine group also.

The difference in the toxicity of aromatic amines requires testing each one to determine its danger.

To evaluate the safety of permanent hair dyes containing PPD, four permanent hair dyes formulas were selected to test their effect on human embryonic kidney HEK293T cell line by Micronucleus assay.

The treatment solutions were prepared by Franz cell so that every hair dye applied on the Franz cell membrane and the solutions found in the receptor chamber were taken for testing by applying four concentrations of them to cell culture.

in hair dye 1 and 2, The results showed a significant increase in micronucleated cells after the treatment and the frequencies ranged between 1.2-3.0 %, whereas the hair dye 3 and 4 didn't appear significant increase in micronucleated cells.

on the mammalian The study concluded that the formulation of hair dye 1 and 2 had genotoxic effects cells in vitro, whereas the formulation of hair dye 3 and 4 didn't have a genetoxic effect and was safe to use.