دراسة مقارنة لتأثير حمض اللينوليك المقترن و زيت جوز الهند البكر على الإنزيمات المضادة للأكسدة و دهون الدم في الجرذان

الملخص EN

The purpose of this study was to evaluate the effect of conjugated linoleic acid (CLA), virgin coconut oil (VCO) and their mixtures on antioxidant enzymes and lipid profile in rats.

Sixty male Wisteralbino rats (eight weeks old and weight 110± 10 g) were randomly assigned to ten groups of six rats in each one.

The first group was fed a control diet containing 4% soy bean oil.

Four groups were fed different concentrations (0.5, 1.0, 1.5 and 2.0%, each increased to 4% using soy bean oil ) of CLA plus soy oil, the other four groups were fed different concentrations of CLA (0.5, 1.0, 1.5 and 2.0%, each increased to 4% using soy bean oil) plus VCO and the last group was fed VCO (4%) alone.

Results showed an increase in the activity of antioxidant enzymes (glutathione reductase, superoxide dismutase and catalase) in rats fed CLA alone and in mixture with VCO, and the differences of the activities of these enzymes were significant (P<0.05) among all groups.

Moreover, such differences were also significant among studied groups and the control group.

The highest activities of these enzymes were noticed in groups fed VCO alone.

CLA, VCO and their mixtures had generally insignificant effects in total cholesterol and highdensity lipoprotein cholesterol (HDL-C) in the serum of rats.

However, VCO alone decreased significantly (P<0.05) the level of low-density lipoprotein cholesterol (LDL-C) and the very low- density lipoprotein cholesterol (VLDL-C) in serum of rats compared to the control group.

On the other hand, the different concentrations of CLA either alone or as mixtures with VCO had no significant effect in these two indicators.

Furthermore, VCO alone or as a mixture with CLA significantly decreased (P<0.05) the concentration of triglyceride (TG) in the serum of rats compared to the control group.

However, the highest decrease in TG was noticed in the group fed VCO alone.

On the other hand, the different concentrations of CLA alone had generally no effect in the concentration of TG in the serum of rats.

Rats fed VCO alone had the highest concentration of malondialdehyde in the serum, whereas the lowest concentration of malondialdehyde was in the serum of the control group and there was a significant difference between these two groups.

Moreover, there was a significant difference in the concentration of malondialdehyde between these two groups and the other groups in this study.

In conclusion, the results suggest that CLA alone or in combination with VCO increased the activity of antioxidant enzymes in serum of rats.

However, the effect of CLA and VCO alone or in combination in plasma lipids was variable