التقدير الفولتامتري للديكساميثازون في مصل الدم و الإدرار و المستحضرات الصيدلانية

الملخص EN

The present work involves a study of the polarographic behaviour of pure Dexamethasone by direct method in aqueous solution , which shows a well defined reduction peaks at a potential (-1.08) V.

in phosphate buffer (pH=7) and (-0.84) V.

in phosphate buffer (pH=3).

The calibration of Dexamethasone was constructed in phosphate buffer (pH=3and7) for the range of concentration (٢.٤٩×10-6M)-(1.54×10-5M)in phosphate buffer (pH=7) with a correlation coefficient (0.9949), and with a range of concentration (6.3×10-7M)-(1.54×10-5M) in phosphate buffer (pH=3) with a correlation coefficient (0.9957).

The calibration curve of Dexamethasone in presence at human blood serum in the range of concentration (3.11×10-6M)-(9.28×10-6M) in phosphate buffer (pH=7) with a correlation coefficient (0.9892), and range of concentration (6.2×10-7M)-(6.21×10-6M) in phosphate buffer (pH=3) with a correlation coefficient (0.9873).

The calibration curve of Dexamethasone in presence of urine in the range of concentration (1.86×10-6M)-(1.53×10-5M) in phosphate buffer (pH=7) with a correlation coefficient (0.9813), and with a range of concentration (6.2×10-7M)-(1.23×10-5M) in phosphate buffer (pH=3) with a correlation coefficient (0.9974).

The method was successfully applied to the determination of Dexamethasone in tablets and syrup in aqueous solution.