التقدير الطيفي للنايترازيبام باقتران الصيغة المختزلة المؤزوتة مع الميتا- امينوفينول ككاشف اقتران

الملخص EN

This paper describes the development of a spectrophotometric method for the determination of microgram amounts of nitrazepam after diazotization of it’s reduced product and coupling with m-aminophenol in basic medium, to form an intense orange – coloured, water – soluble and stable azo-dye which shows a maximum absorption at 478 nm.

Beer’s law is obeyed over the concentration range of 5 to 200μg/25ml with a molar absorptivity 17581.25 l.

mol-1.cm-1 and Sandell’s sensitivity of 0.016 μg.cm-2.

The relative error is ranged from -٢.11 to +1.٤ % and relative standard deviation from ±0.55 to ±2.10 %, depending on the concentration level. The method has been applied successfully to the determination of nitrazepam in pharmaceutical preparation (tablet).