تأثير المطفر أزايد الصوديوم في بقاء الكالس الناتج من أجنة الفاصوليا Phaseolus vulgaris الناضجة

الملخص EN

This research was conducted to study the effect of the chemical mutagen sodium azide on the percentage of callus induction and survival from mature beans embryos Harvester cultivar.

Seeds were treated with the mutagen that was dissolved either in distilled water (pH 6) or in phosphate buffer (pH 3) for 24hrs of 1,2,3 millimolar concentration.

Calli were induced on MS medium in the presence of 0.5 mg/L of Benzyl adenine (BA), lmg/L Indole acetic acid (IAA) and lOOmg/L from each of Casein hydrolysate, Glycine, Asparagine, Tyrosine, and Myo-Inositol.

The results showed that mutagen in water had stimulating effect on callus induction from beans embryo parts at 1, and 2mg/L compared with the control, while the percentage of induction reduced at 3mg/L concentration.

The highest percentage of callus induction (98%) was from hypocotyl treated with 2 millimoler of sodium azide.

Sodium azide in phosphate buffer delayed callus induction and reduced survival rate from all embryo parts compare with the control.

The lowest callus survival rate was for roots treated with 2 or 3mg/L of mutagen that was 5.9%.

Adventitious roots formed on calli surface induced from hypocotyl.

In conclusion this experiment showed that preparation method of sodium azide has major effect on its mutagen ability.

Sodium azide in water increased callus induction while if it dissolved in phosphate buffer it reduced survival rate and thus it is better mutagen.