تأثير طريقة الإكثار الخضري الدقيق و الإكثار التقليدي بالعقل في النمو الحاصل لثلاثة تراكيب وراثية من قصب السكر Saccharum officinarum

الملخص EN

Three genotypes of sugar cane (CO.J.86, CO.J.64 and Missan) were in vitro propagated on MS media using meristem tips.

The effect of different concentrations of BA (0.0, 0.5, 1, 1.5 2 $»nd 2.5mg/L) on shoot multiplication and IAA (0.0, 1, 2, 3, 4 and 5mg/L) on plantlets rooting percentage were investigated.

In addition the effect of a mixture of sand and peat moss was investigated on plants acclimatization and then transfer to soil.

The acclimatized plants and nodes cutting ( of the first year crop) of these genotypes planted in row in the field and observations were made at the end of season to determine the averages of branches number, lengths, fresh weights and also the sugar content in plants.

The result showed that there were significant increase in shoot multiplication in BA (0.5mg/L) in all genotypes for most of characters that were investigated.

The IAA (2mg/L) gave the highest rooting percentage (100 and 83.3%) for CO.J.86 and CO.J.64 genotypes, respectively.

The mixture (l:0.5/v:v) of sand and peat moss gave better results of acclimatization for all genotypes.

The results also showed that plants multiplied by in vitro were significantly increase in the most of characters than plants multiplied using traditional methods (by node cutting) in the field.

The results also showed significant differences between genotypes in the most of characters studied.