إنتاج أنزيم البروتييز من البكتريا المحلية Aeromonas hydrophila المعزولة من مرضى مصابين بالإسهال

الملخص EN

Aeromonas was isolated and diagnosis from clinical sources responsible of causing diarrhea in children, adults and from fish seller wounds.

Two hundred and thirteen isolates showed high productivity of protease by different efficiency.

Semi quantitative screening on a selective solid medium showed that 12 isolates have the ability to produce protease as indicated by the formation of clear zone around the colonies.

The selected isolate was characterized as Aeromonas hydrophila MGAl based on its high production of enzyme and used in the present study.

Results showed that the optimum conditions for protease production by submerged cultures were 1.5% tryptone, 1.5% KH2P04, 0.0002% KC1 with an inoculums size of 8xl07 CFU at an initial pH of 8 after 48 hrs with shaking incubation at 30°C, 150 cycle/min, amounting to 18.72 effective quality unit / mg protein.