Evaluation of transfected HEP-2 cell line using ß-galactosidase reporter assay system

الملخص EN

Liposome-mediated transfection of cancer cells provide a valuable experimental technique to study cellular gene expression and may also be adapted for gene therapy studies.

However, the widely recognized advantage of liposome-mediated transfection is high efficiency.

Therefore, this study were performed to optimize transfection techniques in human larynx carcinoma cell line Hep-2 using the commercial synthetic lipid Trans Fast™ Reagent and monitoring the expression efficiency by using the p SV-β-galactosidase Control Vector which encoded β-galactosidase, maximum transfection efficiency were achieved with Trans Fast™ Reagent used at the Charge ratios of 2:1 and 0.5 µg DNA / ml, this is indicate that Trans Fast™ Reagent can be used as an efficient transfection agent to deliver foreign DNA into human larynx carcinoma cell line Hep-2 and expression of the transgene efficiently.