التشخيص الجزيئي للفطر Verticillium dahliae Kleb المسبب للذبول الفرتسليومي على الزيتون

الملخص EN

The present study was conducted on olive wilt disease caused by Verticillium dahliae Kleb, which has recently distributed in Iraq and is considered as one of the most important and devastating diseases on olive trees in Iraq.

The results of the field survey, carried out in certain regions of Ninevah province during the years 2004 and 2005, showed that the highest disease incidence and severity were found in Alsada region in 2005 and reached 48.72% and 0.29; while the lowest values were recorded in Khorsybad and reached 30.14% and 0.26, respectively.

For the first time in Iraq, the Polymerase Chain Reaction (PCR) was used to diagnose the different isolates of the pathogen using two specific pairs of primers (FVD and RVD).

The PCR results showed that V.dahliae Kleb is the prevalent disease in Iraq which produced DNA fragment bands of 330 bp which was expected for these primers.

The samples were also processed for detection using (NESF18s and NESR28s) primers in Nested PCR where these primers are used in common detection of all Verticillium spp.

and the products were subjected to another PCR reaction using specific primers for V.dahliae (FVD and RVD) .The results of these tests confirmed that V.dahliae is the common species and it was detected in healthy trees (unclear symptoms), indicating that this test is to be used to detect the disease in its early stages.