استعمال تقنية PCR للكشف عن جين Ped المسؤول عن إنتاج Pediocin في الغزلات المحلية لبكتريا Pediococcus acidilactici

الملخص EN

Six isolates were identified to be Pediococcus acidilactici depending on morphological, microscopic examinations and biochemical which tests included negative test for gas production from glucose, production of catalase, reducing of nitrate, citrate utilization, gelatin liquefaction, ammonia production from arginine, esculin hydrolysis, blood haemolysis.

Their resistance to the antibiotic Vancomycin, growth at different concentrations of sodium chloride solution, their ability to growth at 50 ?C and not fermentation of maltose .

The isolate obtained from fermented meat gave the highest production of pediocin because of their high inhibitory direction of the test bacteria.

It was named AK.

The gene which was responsible for pediocin production was identified by using PCR technique .It is found band when 332bp after electrophoresis.