Histological evaluation of platelet–rich plasma effect on bone healing in alloxan-induced diabetic rabbits : Experimental study

الملخص EN

Background: Diabetes Mellitus has an impact on bone healing process, Platelet–\rich plasma PRP ameliorated the deleterious effect of diabetes on bone healing process so the aims of this study were to assess histological the effects of PRP on bone healing in intrabony defect of maxillary bone in alloxan–induced diabetic and nondiabeic rabbits, and to correlate the effect of time of bone healing in two experimental groups with that of controls.

Materials and methods : Fifty rabbits were used, thirty–five rabbits rendered diabetic by the use of alloxan (five of them dead for unknown reasons), the remaining fifteen rabbits left normal.

An intrabony defect was created in the maxilla of each rabbit, the defect of control group left for normal healing, while diabetic rabbits divided2groups First diabetic group, the defect filled with autologous PRP as experimental group -Second diabetic groups, defect left empty.

The specimens were collected at 1, 3, 6 weeks postoperatively.

Histological examination was performed under light microscope for sections stained with heamatoxylline and eosine to assess histomorphometric analysis including, counting of inflammatory cells, fibroblasts, and bone cells infiltration.

The regeneration levels were detected by measuring the thickness of new bone trabeculae by a special graduated lens, at the end of each three periods.

Results: Histological examinations showed that with the use of autologous Platelet–rich plasma in a bone of diabetic rabbit, an obvious enhancement of new bone forming and neovascularzation significantly more than that of diabetic group without PRP application, the results of diabetic group treated with PRP reached the levels of normal group in all the three periods postoperatively.

Conclusions : this study illustrated that PRP has an osteopromotive activity that accelerated bone healing process in maxillary bone of diabetic rabbits.