Factors affecting the production of β-galactosidase in different strains of enterobacter aerogenes
العناوين الأخرى
العوامل المؤثرة في إنتاجية إنزيم بيتا-جالكتوسيديز في أنواع مختلفة من enterobacter aerogenes
مقدم أطروحة جامعية
al-Shurafa, Khalid Yusuf Ahmad
مشرف أطروحة جامعية
أعضاء اللجنة
Khulayfat, Khalid Muhammad
al-Shawabikah, Riyad
Kanan, Ghassan Jaddu
الجامعة
جامعة مؤتة
الكلية
كلية العلوم
القسم الأكاديمي
قسم الأحياء
دولة الجامعة
الأردن
الدرجة العلمية
ماجستير
تاريخ الدرجة العلمية
2004
الملخص الإنجليزي
Growth conditions which were expressed as biomass or OD60o nm as well as the production of p-galactosidase enzyme were investigated in different strains of Enterobacter aerogenes.
Three Enterobacter strains were included in this studies and there are pUC8:16 ; A transformant carrying Vitreoscilla hemoglobin gene (vgb) and two control strains.
The first (pUC9) is a transformant carrying the plasmid only without vgb while the other control represents parental cells.
The optimum growth conditions were not totally identical to these for the enzyme production.
PH 7 and agitation rate of 150 rpm were common for both measurements.
However the optimum temperature for growth was approximately 37°C while that for P-galactosidase synthesis was approximately 42°C.
The latter temperature gave almost five fold higher rate of enzyme production over the 37°C.
The bacterial growth was relatively stable within a range of 0.08-0.5 % NaCl supplement while the enzyme synthesis was severely repressed.
The effect of five different nitrogen sources (three organic and two inorganic) was also investigated either by using various concentrations of each nitrogen supplement or under fixed concentration of different nitrogen sources.
It was found that all used nitrogen sources either induce or have little effects on bacterial growth.
In contrast the p-galactosidase production was repressed in all Enterobacter strains through more severely in presence of inorganic nitrogen supplements.
The VHb protein in the transformed Enterobacter pUC8:16 strains had shown a protective actions by minimizing the enzyme repression caused by salt or nitrogen supplements.
التخصصات الرئيسية
عدد الصفحات
78
قائمة المحتويات
Table of contents.
Abstract.
Chapter One : statement of the problem.
Chapter Two : material and methods.
Chapter Three : finding.
Chapter Four : discussion, conclusions and recommendations.
References.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
al-Shurafa, Khalid Yusuf Ahmad. (2004). Factors affecting the production of β-galactosidase in different strains of enterobacter aerogenes. (Master's theses Theses and Dissertations Master). Mutah University, Jordan
https://search.emarefa.net/detail/BIM-297693
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
al-Shurafa, Khalid Yusuf Ahmad. Factors affecting the production of β-galactosidase in different strains of enterobacter aerogenes. (Master's theses Theses and Dissertations Master). Mutah University. (2004).
https://search.emarefa.net/detail/BIM-297693
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
al-Shurafa, Khalid Yusuf Ahmad. (2004). Factors affecting the production of β-galactosidase in different strains of enterobacter aerogenes. (Master's theses Theses and Dissertations Master). Mutah University, Jordan
https://search.emarefa.net/detail/BIM-297693
لغة النص
الإنجليزية
نوع البيانات
رسائل جامعية
رقم السجل
BIM-297693
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر