إكثار و تضعيف فايروس الحمى القلاعية بتمريره في أجنة الدواجن

الملخص EN

Thiis sttudy iinclludes replliicattiion and attttenuattiion of foott and moutth diisease viirus ttype O whiich iisollatted from iinfectted callves..

Many passages for tthe viirus iin chiick-Embryo were esttablliished as a substtiittutte metthod tto tthe ttiissue culltture whiich iis hiighlly causttiic iin conttrastt tto tthe chiick embryo..

The viirus passed tten consequentt passages whiich llead tto tthe reduce of tthe ttiitter of tthe viirus from 106..53 TCID50// 0..1 mll iin cattttlle ttesttiis ttiissue culltture tto 103 TCID50// 0..1 mll..

tthe patthogeneciitty of attttenuatted FMD viirus were allso sttudiied iin botth chiick-embryo and guiinea piigs..

Usiing agar gell diiffusiion ttestt preciipiittattiion anttiibodiies was dettectted iin guiinea piig serum aftter 14 and 21 days postt exposure tto tthe attttenuatted viirus..

The iinocullatted guiinea piig group wiitth tthe chiick-embryo attttenuatted viirus appear resiisttance tto tthe challllenge viirus..

The resulltt suggestted tthe effiicacy of attttenuattiion of foott and moutth diisease viirus by usiing chiick-embryo systtem for iimmuniizattiion agaiinstt tthiis diisease on tthe llevell of llaborattory Aniimall.