تأثير مستخلصات أزهار البيضاء لنبات الداوودي Chrysanthemum cineraiaefolium على حشرة خنفساء الطحين Tribolium castanum

الملخص EN

The production, purification and determination of (Pyrethrin I, II) from the white flowers of Chrysanthemum cineraiaefolium plant were performed.

The results obtained was (450 ml) as concentrated crude extract from using (25gm) powdered flowers dissolved in (1300ml) hexane.

This extract was divided in to three parts, the first part (A) was the concentrated crude extract which have the yellow-greenish colour, sticky and sharp odder, while the second part (B) was the (A) extract dissolved in (methanol 80 %) which have pale–yellow colure and the third part (C) was the (B) extract with the activated charcoal, which was colourless extract.

The results of HPLC analysis for determination, purification and quantity assessment for the three parts (A, B, C) were (73.33 %, 77.33 %, 91.41 %) respectively, and the concentration rate of (Pyrethrin I) to (Pyrethrin II) reached nearly (70 and 30) respectively for the (A, B, C) extracts.

The biological activity test for the two compound mentioned above was carried out as insecticide for repellence and killing the flour beetles during different times (minutes) of treatment, using seven different concentrations of this extracts.

The results obtained show that when increasing of the concentration and the time of treatment, the killing percentage was increased, such as in the concentration {(1: 0.5)–(crude : alcohol)} during (10 minutes) was 98 % while, after (20 min) became 100 %.