The usage of ß-glucan which extracted from Saccharomyces cerevisiae yeast cells wall as immunomodulator

الملخص EN

Carbohydrates (polysaccharides) were extracted from Saccharomyces cerevisiae cell wall by hot alkaline solution, the polysaccharides concentration was 96 mg / ml, while protein concentration was 500 μg / ml, then primary purification method was done to separated carbohydrates from other protein materials, at this time carbohydrates concentration was 250 mg / ml, while protein concentration was 148 μg / ml .Then partial purification using Ion exchange technique was done, and the results revealed, four peaks were separated, every peak was collected, dialysed, and measured the carbohydrates concentration which were 300, 50, 33.4 and 32 mg / ml respectively, while Lowery method did not detect any protein materials in samples.

The four peaks were examined as immunomodulation through the animal (mice) that were divided into 4 groups which depend upon the peaks series, were injected intraperitoneal and orally administration with constant concentration 500 μg / ml twice, then read the blood picture for immune cells, the second group was reported a significant increasing in all cells which are (2.968 ± 0.316), (1.56 ± 0.3), (0.546 ± 0.291).

Lymphocyte, Granulocytes and monocyte respectively while the cell percentage for first, third, and fourth peaks were for lymphocyte (2.292 ± 0.3) (1.83 ± 0.32) and (2.67 ± 0.5) respectively, for Granulocytes (0.83 ± 0.23) (0.22 ± 0.53) and (0.6 ± 0.35) respectively, while for Monocytes (0.3 ± 0.19) (0.01 ± 0.03) and (0.02 ± 0.3) respectively when compared with control group.

Organs crossly examination was showed hypertrophy of liver and spleen, while microscopically, there were infiltration of lymphocytes around the blood vessels in liver, lung, and peritonea, and in white pulp of spleen.