دراسة وراثية و بكتريولوجية حول البكتريا الهوائية المسببة لتلوث جروح العمليات القيصرية لدى النساء في مدينة الديوانية

الملخص EN

This study was designed to stand on the most important causes of contamination in sesarean section in women by the aerobic bacteria sp ecies the bacteria isolates were diagnosed in the Maternity and Children Hospital in the Qadsiya province and in the sp ecial clinics.

Sixty five persent of women having cesarean operation were contaminated with different bacteria in different ratios.

Staphylococcus aureus showed the higher percentage (62.4) , Pseudomonas.aerogenosa 18% , E.coli 9.5% and Proteus.spp (6,4%).While Streptococcus pyogens showed lowest percentage 3.6% .

The results of antibiotic sensitivity test for all bacteria isolates showed Chloramphenicol and Amikacin where efficient in it effect on bacteria , while Amoxicillin and Ampcillin where low efficient in there effect .

About antibiotic combination test on Staphylococcus aureus and Pseudomonas aerogenosa the results showed sy neergstic effect between Amoxicillin and Gentamycin and cefotaxin.

The results of electophoresis of DNA that extracted from sp ecies of isolated bacteria, showed that E.coli Pseudomonas.aerogenosa and Proteus spp one plasmid band while Staphylococcus contain two small plasmid band and there is no relation ship between the number of bands and the resistance for antibiotics.