إنتاج مصل مضاد للكشف عن الفطر Pythium aphanidermatum باختبار اليزا

الملخص EN

Cucumber plant Cucumis sativus L.

is known to be affected by many diseases including root rot and damping off caused by the fungus Pythium aphanidermatum.

To speed up the identification process, this study was carried out to develop an immunological method to detect this pathogen by DAS-ELISA.

Fungal proteins were extracted, and three subcutaneous injections were administered in to a white rabbit with 0.5 mg/injection.

Antiserum against fungal proteins was obtained, and the immunoglobulin G (IgG) fraction was purified and conjugated with alkaline phosphatase to use for detection by DAS-ELISA.

The ELISA value of protein extracted from P.

aphanidermatum was 1.32 whereas the value was 0.22 for the protein extract of Alternaria solani and 0.24 for protein extract of Rhizoctonia solani.

The results obtained demonstrate the high specificity of the antibodies produced for detecting P.

aphanidermatum by DAS-ELISA.

Accordingly, this test can be used as complementary test to the classical methods to enhance diagnosis speed and accuracy.