زيادة كفاءة القدرة التخصيبية في عمليات التلقيح الاصطناعي و الحقن المجهري لمرضى العقم المصابين بوهن النطف الحاد باستخدام الوسط FertiCult Flushing Medium

الملخص EN

This study was included examination and activation of 45 samples for asthenozoospermic men.The research was aimed to improvement of infertility capacity and activation sperm parameters in vitro by using FertiCult flushing medium ,three techniques (swim up-centrifugation wash-out-mixing after washing and centrifugation).The result was revealed significant increase (p<0.05) of sperm motility percent ,grade of sperm activatiy and sperm motility index so significant decrease (p<0.05) of sperm concentration ,non normal sperm morphology percent and leukocytes concentration after using the medium and swim up in 60 min .

In ICSI programs by using the culture medium and centrifugation and swim up technigue with incubation period (45 min.), the results were showed a significant increase (P<0.05) of sperm motility percent, grade of sperm activity , sperm motility index and normal sperm percent so it revealed significant decrease (P<0.05) of sperm concentration and leucolyte concentration in comparison to that of before activation and after activation incubation periods are 5-30 min.

In conclusion,the FertiCult flushing medium influence of the result after activation and increase of improvement the chance in vitro fertilization