التقدير الطيفي للنايترازيبام باستخدام الفلوروكلوسينول ككاشف اقتران أزوتي

الملخص EN

This paper describes the development of a spectrophotometric method for the determination of nitrazepam after diazotization of it’s reduced product and coupling with phloroglucinol in basic medium, to form an intense yellow–coloured, water–soluble and stable azo-dye which shows a maximum absorption at 434 nm.

Beer’s law is obeyed over the concentration range 15 to 200 μ g / 25 ml with a molar absorptivity 6.1183 × 104 l.

mol- 1.cm-1 and Sandell’s sensitivity of 0.0046 μ g.cm-2.

The relative error is ranged from -1.6 to 2.8 % and relative standard deviation from ± 0.1 to ± 3.3 %, depending on the concentration level.

The method has been applied successfully to the determination of nitrazepam in pharmaceutical preparation (tablet).