فعالية و خواص أنزيم 5-نيوكليوتايديز في السائل الأمنيوني البشري

الملخص EN

The present research includes a study of activity and properties of 5 \ -Nucleotidase (5\ -NT, EC.3.1.3.5) was determined in human amniotic fluid using coloremetric method.

Maximal activity of the enzyme was obtained in a reaction mixture containing 40 mM of Barbiturate-HCl buffer at pH 7.5 containing 11 mM of adenosine 5 \ -monophosphate (5 \ - AMP) as a substrate and 50 μl of amniotic fluid (Enzyme volume).

The reaction mixture was incubated at 37°C for 30min, under the optimum condition.

The enzyme activity equal to (66.7 ± 0.32) unit per liter in amniotic fluid.