تأثير نترات الأمونيوم و البوتاسيوم في بعض صفات كالس و أجنة نخيل التمر Phoenix dactylifera L. صنف البرحي خارج الجسم الحي

الملخص EN

The present study was undertaken at Tissue Culture Laboratory-Date Palm Research Center of Basrah University from January 2007 to February 2008 to determine the effect of Ammonium and Potassium nitrate on some characteristics of callus and somatic embryos of date palm cv.

Barhee by in vitro.

Four concentrations of potassium nitrate were used (zero, 1 / 2, full and double strength) and three concentrations of Ammonium nitrate were used (zero, 1 / 2, full strength) the results showed that addition the potassium nitrate at a concentration of full strength and ammonium nitrate at concentration of 1 / 2 strength led to significant increase in the average of fresh weight of embryogenic callus, number of somatic embryos (354.33,321.75 mg / gm fresh weight) respectively, while number of somatic embryos was 10.94,11.17 embryos respectively and the results showed that the percentage of vitrification were decreased in the media supplemented with half strength of potassium nitrate 26.37 % also the percentage of vitrification were decreased in the media supplemented with free ammonium nitrate 22.29 %.

The best percentage of vitrification were in the media supplemented with half strength of potassium and ammonium nitrate 15.24 %.

At the last the results showed that the total soluble phenols were decreased in the media supplemented with little concentration of potassium and ammonium nitrate, it reach 0.18 mg / gm dry weight in the media supplemented with half strength of potassium nitrate, while reach 0.18 mg / gm dry weight in the media supplemented with half strength of ammonium nitrate.

The best result was obtained in the media that supplemented with half strength of potassium and ammonium nitrate it reach 0.15 mg / gm dry weight.