Cloning and sequence analysis of lipl32, a surface–exposed lipoprotein of pathogenic leptospira spp
المؤلفون المشاركون
Yahaghi, Imad
Darian, Ibrahim Khodaverdi
Forghanifard, Muhammad Mahdi
Bidhendi, Suhaylah Muradi
Chang, Yung-Fu
Esmaelizad, Majid
Khaleghizadeh, Maryam
Khaki, Pejvak
المصدر
Iranian Red Crescent Medical Journal
العدد
المجلد 15، العدد 11 (30 نوفمبر/تشرين الثاني 2013)15ص.
الناشر
تاريخ النشر
2013-11-30
دولة النشر
الإمارات العربية المتحدة
عدد الصفحات
15
التخصصات الرئيسية
الموضوعات
الملخص EN
Background : Leptospirosis is a worldwide zoonosis caused by pathogenic Leptospira species.
A major challenge of this disease is the application of basic research to improve diagnostic methods and related vaccine development.
Outer membrane proteins of Leptospira are potential candidates that may be useful as diagnostic or immunogenic factors in treatment and analysis of the disease.
Objectives : To develop an effective subunit vaccine against prevalent pathogenic Leptospira species, we sequenced and analyzed the LipL32 gene from three different Leptospira interrogans (L.interrogans) vaccinal serovars in Iran.
Materials and Methods : Following DNA extraction from these three serovars, the related LipL32 genes were amplified and cloned in the pTZ57R / T vector.
Recombinant clones were confirmed by colony-PCR and DNA sequencing.
The related sequences were subjected to homology analysis by comparing them to sequences in the Genbank database.
Results : The LipL32 sequences were > 94 % homologous among the vaccinal and other pathogenic Leptospira serovars in GenBank.
This result indicates the conservation of this gene within the pathogenic Leptospires.
Conclusions : The cloned gene in this study may provide a potentially suitable platform for development of a variety of applications such as serological diagnostic tests or recombinant vaccines against leptospirosis.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Darian, Ibrahim Khodaverdi& Forghanifard, Muhammad Mahdi& Bidhendi, Suhaylah Muradi& Chang, Yung-Fu& Yahaghi, Imad& Esmaelizad, Majid…[et al.]. 2013. Cloning and sequence analysis of lipl32, a surface–exposed lipoprotein of pathogenic leptospira spp. Iranian Red Crescent Medical Journal،Vol. 15, no. 11.
https://search.emarefa.net/detail/BIM-343100
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Darian, Ibrahim Khodaverdi…[et al.]. Cloning and sequence analysis of lipl32, a surface–exposed lipoprotein of pathogenic leptospira spp. Iranian Red Crescent Medical Journal Vol. 15, no. 11 (2013).
https://search.emarefa.net/detail/BIM-343100
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Darian, Ibrahim Khodaverdi& Forghanifard, Muhammad Mahdi& Bidhendi, Suhaylah Muradi& Chang, Yung-Fu& Yahaghi, Imad& Esmaelizad, Majid…[et al.]. Cloning and sequence analysis of lipl32, a surface–exposed lipoprotein of pathogenic leptospira spp. Iranian Red Crescent Medical Journal. 2013. Vol. 15, no. 11.
https://search.emarefa.net/detail/BIM-343100
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-343100
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر